One-step growth

Draw the general progression of step growth polymerization. Draw a specific example of the reactions to make one step growth polymer. 

Figure 1.10 The one-step growth curve of virus replication. This graph displays the results of a single round of viral multiplication in a population of cells.

Figure 5.32 One-step growth curve of animal viruses.

Extracts that exhibited significant inhibitory activity, defined as >50% inhibition of CPE at 100 (xg/mL, were advanced into secondary screening, which includes confirmation of activity observed during the primary screen using an expanded range of concentrations (dose response), plaque inhibition assay, and a one-step growth inhibition and testing of additional influenza viruses. As shown in Fig. 1.2a, the quantitative dose response assay was used to assess the potency of the most two... 

In order to study the virus growth curve a one-step growth cycle is performed. A high multiplicity of infection (m.o.i.) is used to ensure every cell is infected — usually 10 plaque forming units (p.f.u.) per cell is adequate. For virus production, however, the infection is prolonged under conditions where secondary infection can occur and a low m.o.i. is recommended especially where there is a tendency for defective virus particles to be produced. 

Proctor, L. M., Okubo, A., and Fuhrman, J. A. (1993). Calibrating estimates of phage-induced mortality in marine bacteria Ultrastmctural studies of marine bacteriophage development from one-step growth experiments. Microb. Ecol. 25, 161-182. 

Two assays were developed that measure the potency of the FGF-4 transgene carried by Ads FGF-4. In the first case, a one-step growth-promotion assay is conducted on normal, human retinal pigment epithelial cells (ARPE-19). The assay measures metabolic activity (Alamar blue dye metabolism) following infection of ARPE-19 cells with a serial dilution of the virus. The increase in metabohc achvity was measured in relation to a mock-infected control. This increase correlates with FGF-4 produchon determined by an FGF-4 ELISA, increased de-novo DNA synthesis measured by BrdU incorporation, and an increase in cell number. This procedure is therefore an appropriate in-vitro efficacy measure, indicating that the FGF-4 transgene product is biologically active. 

Fig. 6.8 One-step growth-promotion assay. Mock-infected ARPE-19 cells (A) and ARPE-19 cells infected with the negative control AdSLacZ (B) or Ad5FGF-4 (C) on day 1 post infection. (Original magnification, x4.)...

The importance of the conclusion that bacterial multiplication stops should not be ignored. This fact has since been affirmed by colony counts of bacteria multiply infected with T2r+ (54), T3 (225), and T7 (262), by cytochemical observation, and by the constancy of the infectious centers during the latent period of one-step growth curves of multiply infected bacteria. Indeed, no one has ever demonstrated that phage-infected E. coli can multiply. ... 

Figure 10.25 SEM images of the laterally overgrown GaN layer using one-step growth at 1050 °C (a) plan-view image of the layers after short time of growth (0.5 hour). There is no coalescence between the wings ...

Biirk, R. R., 1970, One-step growth cycle for BHK21/13 hamster fibroblasts, Exp. Cell Res. 63 309. 

Tsvetkova, I. V., and Lipkind, M. A., 1973, Studies on the role of myxovirus neuraminidase in virus-cell receptor interaction by means of direct determination of sialic acid split from cells. 3. One-step growth kinetics of accumulation of the sialic acid liberated from NDV-infected chick embryo cells. Arc/ . Gesamte Virusforsch. 42 125. 

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