Oligonucleotide-based

M. Smith (University of British Columbia) fundamental contributions to the establishment of oligonucleotide-based, site-directed mutagenesis and its development for protein studies. 

Kucho, K.-I., Y. Tsuchiya, Y. Okumoto et al. (2004). Construction of unmodified oligonucleotide-based arrays in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 Screening of the candidates for circadianly expressed genes. Genes Genet Syst 79 319-329. 

Ying Z, Xiang-Ying S, Bin L (2009) Fluorescent recognition for single- and double-stranded oligonucleotides based on rhodamine B-modified self-assembled bilayers. Chin J Anal Chem 37 665-670... 

Oligonucleotide-based drugs such as antisense drugs, aptamers, and small interfering RNA (siRNA) have attracted considerable attention as promising therapeutic agents for the treatment of various human diseases [59], To develop... 

Heckelsmiller, K. et al., Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy, Eur. J Immunol, 32, 3235, 2002. 

Example 43 Michalski et al. have described a synthetic approach to modified oligonucleotides based on iV,Ar-diisopropyl-di-(4-nitrophenyl)phospho-roamidite. The procedure involves displacement of either the 4-nitrophenoxy or the diisopropylamino ligand [78]. 

H. Hess-Stumpp, S. Klussmann, In vivo properties of an anti-GnRH Spiegelmer An example of an oligonucleotide-based therapeutic substance class, Proc. Nat. Acad. Sci. USA 99 (2002) 8898-8902. 

An array of oligonucleotide which is composed of 20 80-mer oligos, or peptide nucleic acid probes, is synthesized either in situ (i.e., on-chip) or using conventional synthesis followed by on-chip immobilization. The resultant DNA array is then exposed to the labeled sample of DNA, hybridized, and the identity/abundance of complementary sequences determined. This method was developed at Affymetrix, Inc. and called DNA chips. Today, oligonucleotide-based chips are manufactured by many companies using alternative in situ synthesis or depositioning technologies. 

Morishita R, Kaneda Y Ogihara T, Therapeutic potential of oligonucleotide-based therapy in cardiovascular disease, Bio Drugs 2003 17(6) 3 83-3 89. 

Matsuki T, Hori G, Furuichi T. Gene expression profiling during the embryonic development of mouse brain using an oligonucleotide-based microarray system. Brain Res Mol Brain Res 2005 136(l-2) 231-254. 

Driver SE, Robinson GS, Flanagan J, Shen W, Smith LEH, Thomas DW, Roberts PC. Oligonucleotide-based inhibition of embryonic gene expression. Nat Biotech-nol 1999 17 1184-7. 

Contemporaneously with these recent discoveries, our laboratory has used directed evolution by random sequence mutagenesis of a region of human TS near the catalytic cysteine (position 195) to produce novel 5-FU resistant TS mutants. A plasmid-encoded library of TS mutants was constructed using an oligonucleotide-based random mutagenesis technique. The end product of this process was a family of over 2 million... 

Maynard C, Berthiaume F, Lemarchand K, Harel J, Payment P, Bayardelle P, Masson L, Brousseau R (2005) Waterborne pathogen detection by use of oligonucleotide-based microarrays. Appl Environ Microbiol 71 8548-8557... 

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