Oligonucleotide-based therapeutics

H. Hess-Stumpp, S. Klussmann, In vivo properties of an anti-GnRH Spiegelmer An example of an oligonucleotide-based therapeutic substance class, Proc. Nat. Acad. Sci. USA 99 (2002) 8898-8902. 

Riorden, M L and Martin, J. C. (1991) Oligonucleotide-based therapeutics Nature 350,442A43,... 

Nonionic oligonucleotide analogs have attracted much interest in recent years as potential candidates for oligonucleotide-based therapeutics. Compared to their natural parent molecules, they are expected to have the advantage of ... 

Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and antisense oligonucleotide -based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. 

By similar logic, protein affinity Hbraries have been constmcted to identify protein—protein combining sites, as in antibody—antigen interaction (19) and recombinant Hbraries have been made which produce a repertoire of antibodies in E. coli (20). In another case, a potential DNA-based therapeutic strategy has been studied (21). DNAs from a partially randomized Hbrary were selected to bind thrombin in vitro. Oligonucleotides, termed aptamers that bound thrombin shared a conserved sequence 14—17 nucleotides long. 

Oligonucleotide-based drugs such as antisense drugs, aptamers, and small interfering RNA (siRNA) have attracted considerable attention as promising therapeutic agents for the treatment of various human diseases [59], To develop... 

Morishita R, Kaneda Y Ogihara T, Therapeutic potential of oligonucleotide-based therapy in cardiovascular disease, Bio Drugs 2003 17(6) 3 83-3 89. 

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