Oligonucleotides based drug

Oligonucleotide-based drugs such as antisense drugs, aptamers, and small interfering RNA (siRNA) have attracted considerable attention as promising therapeutic agents for the treatment of various human diseases [59], To develop... 

Liposomes have been investigated in the delivery of the gene-based drugs for more than a decade. The cellular transfer of genes and oligonucleotides is obtained with cationic lipo-... 

Peptides have been used for the delivery of gene-based drugs. Several rationales have been utilized. Cationic peptides are used to neutralize the charges of DNA or oligonucleotide. These peptides include polylysines and polyarginines [241,244,245]. They form complexes with DNA or oligonucleotide, thereby resulting in enhanced cellular uptake. 

Morishita R, Kaneda Y Ogihara T, Therapeutic potential of oligonucleotide-based therapy in cardiovascular disease, Bio Drugs 2003 17(6) 3 83-3 89. 

Among DNA-based drugs, only antisense DNA is currently analyzed by capillary electrophoresis. Antisense DNA therapy requires reliable and convenient methods for sequencing short single-stranded oligonucleotides. A method for phosphorothioate antisense DNA sequencing by capillary electrophoresis coupled to UV detection has been developed based on a modified chain-termination sequencing method.Capillary gel electrophoresis alone or in combination with HPLC is also used for pharmacokinetics study of antisense... 

The identification of DNA as a primary target for metal-based drugs, especially cisplatin, has focused attention on the interactions of metal complexes with nucleic acid constituents, which include the simple purine and pyrimidine bases and their nucleoside and nucleotide derivatives. The structures, with abbreviations, are represented in Appendix 1. Simple complexes can represent models for cross links in DNA, which can be studied in more detail with small polynucleotides, from the simpler dinucleotides to oligonucleotides and this topic is covered in Section 4.4. There has been extensive use of substituted purines and pyrimidines as models for the DNA bases and in the examination of steric and electronic effects. The structures of many of these analogues are also collected in Appendix 1. 

Besides CpG ON cargoes, DNA nanostructures have been also utilized to deliver other NAs-based drugs such as antisense oligonucleotides. 

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