Nylon-supported , membrane

The nature of the supporting membrane also plays an important role in the performance of supporting ionic liquid membranes. In this context, de los Rios et al. [3] nsed two polymeric membranes, nylon and mitex, as supporting membranes. Nylon membrane was a hydrophilic polyamide membrane with a pore size of 0.45 pm and a thickness of 170 pm. Mitex membrane was a hydrophobic polytetrafluoroethylene membrane with a pore size of 10 pm and a thickness of 130 pm. It was observed that less ionic liquid was absorbed into the mitex membranes, which was explained by the different textural properties and the high hydrophobic character of these membranes, which probably restrict interaction with the hydrophilic ionic liquids used [27]. 

Blotting The transfer of proteins or nucleic acids from an electrophoresis gel to a manbrane support (such as nitrocellulose or nylon). The membrane blot is then incubated with probes that bind the molecules of interest. 

The supported ionic liquid membranes (SILMs) were prepared by depositing the ionic liquids [hmim][Tf2N] and [H2NC3H6mim][Tf2N] on top of cross-linked nylon support discs in a shallow glass container. A sufficient amount of ionic liquid to cover the membrane was used. The membrane was allowed to absorb the ionic liquid for at least 4 hours, and then the SILMs were removed from container and blotted dry. Further details concerning this procedure were published earlier [19]. 

Dye Chitosan membrane on nylon support Laccase Katuri et al., 2009... 

Quaternary ammonium (3) and phosphonium ions (61), crown ethers such as (62), cryptands such as (63) and poly(ethylene glycol) ethers (64) bound to PS are catalysts for reactions of water insoluble organic compounds with organic insoluble inorganic salts. " Silica gel, alumina, polystyrene-polypropylene composite fibers, nylon capsule membranes, and polyethylene (Mn 1000-3000) have also been used as supports. The reactions are called phase-transfer-catalyzed because one or both of the reactants are transported from the normal liquid or solid phase into a polymer phase, where the reaction proceeds. 

Membrane cartridge filters are extremely flexible and high in tensile strength. The cartridge construction is based on a multi-layer combination of filter media in pleated format. Those polymers that have been used extensively as filtration media in coarser grades are now widely used as membrane filters. A typical format has a cartridge fabricated from a pleated filter pack, which contains a very fine polyolefin fibre prefilter layer, two nylon membranes of the same pore (0.2 pm) size, and a downstream polypropylene support. The layers of nylon microporous membrane and polypropylene prefilter are pleated together and snpported by an inner snpport core. The end-caps and core are melt sealed in polypropylene. 

Moreover, the unique adsorption properties of GEC allowed the very sensitive electrochemical detection of DNA based on its intrinsic oxidation signal that was shown to be strongly dependent of the multi-site attachment of DNA and the proximity of G residues to GEC [100]. The thick layer of DNA adsorbed on GEC was more accessible for hybridization than those in nylon membranes obtained with genosensors based on nylon/GEC with a changeable membrane [99,101,102]. Allhough GEC has a rough surface, it is impermeable, while nylon is more porous and permeable. DNA assays made on an impermeable support are less complex from a theoretical standpoint [7] the kinetics of the interactions are not compUcated by the diffusion of solvent and solutes into and out of pores or by multiple interactions that can occur once the DNA has entered a pore. This explained the lower hybridization time, the low nonspecific adsorplion and the low quantity of DNA adsorbed onto GEC compared to nylon membranes. 

Membranes such as NC supported on glass may be more applicable for protein microarrays than glass substrates. Supported charged nylon membranes for microarrays are currently entering the marketplace as well. The essential ingredient for protein is water. Protein hydration reduces the likelihood for surface denaturation. Hydrophilic membranes allow proteins to... 

The binding of an antibody to its antigen has also been utilized in the detection of specific proteins on a solid support, such as a western blot, where size-separated proteins are immobilized on a nitrocellulose or nylon membrane (see Section V.B). In this case, the primary... 

AP-based luminescent protocols that achieve maximum sensitivity with minimum background can be complex, and the manufacturer s instructions should be consulted (see Reagents and Solutions). The procedure described in Support Protocol 2 gives reasonable sensitivity on nitrocellulose, PVDF, and nylon membranes with a minimum of steps. 

One of the most extensively applied techniques to retain and reuse the enzyme in continuous operation is the immobilization onto a support. The outlet port is provided with a nylon membrane to retain the solid biocatalyst inside the... 

Enzymes have been attached to a nylon matrix (135-13 7), a pig intestine (138), the hydrophobic membrane of a gas-selective sensor (139), and controlled pore glass (140). Recent comparative studies of the coupling agents GA and benzoquinone support the preferential use of benzoquinone for binding GOD to nylon mesh (13 7), or to cellulose acetate membranes (141) with lysine (13 7). Both investigations report robust electrode behaviors with respect to prolonged exposure to glucose, while lifetimes of the membrane electrodes were ca. 3 months. 

Abdel-Hamid et al. [122] used a flow-injection amperometric immunofll-tration assay system for the rapid detection of total E. coli and Salmonella. Disposable porous nylon membranes served as a support for the immobilization of anti- ]. coli or anti-Salmonella antibodies. The assay system consists of a flow-injection system, a disposable filter-membrane, and an amperometric sensor. A sandwich immunoassay specifically and directly detected 50 cells ml total E. coli or 50 cells ml Salmonella. The immunosensor can be used as a highly sensitive and automated bioanalytical device for the rapid quantitative detection of bacteria in food and water. 

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