Nutrient Agar plate

Resistance Tests. Several transformants were tested for their ability to grow on all three selective media. These transformants were streaked onto nutrient agar plates containing 0.1% naphthalene, dibenzofuran, or... 

The intensive search for new organic fungicides has continued for over 30 years. The technique employed in most laboratories is to make an extensive survey of various structures by empirical methods to locate materials that suppress spore germination on glass slides or prevent mycelial growth on nutrient agar plates or rolled tubes. Those materials having an ED ] (effective dose for 50% inhibition) in (he order of 10 ppm are further tested in use applications. 

Cultures of the bacteria were streaked on nutrient agar plates containing various concentrations of the honeys, and the growth of the bacteria was assessed to find the concentration of honey that... 

A sample of raw river water was spiked with two loopfuls of Klebsiella oxtyoca. 100 ml aliquots of this of this spiked water solution were brought to 0.05 ppm, 0.1 ppm, 0.2 ppm, 0.5 ppm, or 1.0 ppm of inventive silver composition. After an incubation of 5-60 minutes, the samples were membrane filtered. The filter was rinsed with approximately 100 ml sterile water. The filters were aseptically removed from the filter housing and placed on coliform nutrient agar plates. The plates were incubated under growth conditions for 24 hours and counted. 

Numbers within parentheses indicate number of colonies cured of resistances out of 100 colonies tested broth cultures after treatment with Th were plated out for isolated colonies which were then transferred to different antibiotic agar and nutrient agar plates. These plates contained 25% of the MIC of a particular antibiotic with respect to the given type (untreated). 

B. anthracis cells are lysed by the bacterial virus y-bacte-riophage to form small plaques on nutrient agar plates. 

However, more meaningful data can be obtained if force rather than gravity is used to collect airborne particles. A stream of air can be directed onto the surface of a nutrient agar plate (impaction slit sampler) or bubbled through an appropriate buffer or culture medium (liquid impingement). Various commercial impactor samplers are available. Filtration sampling, where the air is passed through a porous membrane, which is then cultured, can also be used. 

Culture on nutrient agar plates containing ampicillin... 

The isolate 9B, purified from the mixed culture enriched in the presence of di-n-tridecyl sulfosuccinate, was identified as Pseudomonas sp. on the basis of the following results. Morphology on nutrient agar plates circular, entire, smooth, shiny umbonate colonies 2-4 mm in diameter. Positive tests catalase, acid from glucose growth at 37°C,... 

Geotrichum candidum was obtained from Birkbeck College Culture Collection and maintained on nutrient agar plates with 2-monthly transfers. 

We recently surveyed a cross-section of plants from many tropical regions of the world in a search for photosensitizers to further test the above hypothesis. The methods used to test for phototoxic phytochemicals are described in detail elsewhere (22). Briefly, methanolic extracts were spotted onto sterile filter-paper discs and allowed to dry. The dried discs were placed onto replicate nutrient agar plates that had been spread with Ex B/r (a UV resistant bacterium). The plates were incubated in the dark at 3TC for 30 min. Half of the plates were irradiated for 60 min. with eight Sylvania F40BLB UVA lamps (18W m ), while the other half were kept in the dark. All plates were incubated overnight in the dark at 37°C, after which the zones of inhibition surrounding the filter paper discs were measured. 

The agar difihision test is used to qualitatively assess the efficacy of textiles treated with diffijsible biocides. Samples are plamd in the centre of nutrient agar plates which have been inoculated widi the test bacteria. The samples are incubated at 37°C for 18-24 hours. The evaluation of this test is based on the level of growth both under and around the sanqrie. (Fig. 2) The zone of inhibition around the test material is measured and any growdi present underneath the sample is scored. 

---