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Nurse cell development

Development in Drosophila and other insects follows a somewhat different pathway, as is indicated in Fig. 32-6B. The egg, which is surrounded by follicle cells and 16 nurse cells, does not divide. However, its nucleus divides repeatedly, about once every nine minutes, to form -6000 nuclei. Only then do separating membranes form to give individual cells.9,285 316 317 During the first two hours the nuclei form a syncytium, in which they are embedded in a common cytoplasm, that allows free diffusion of signaling compounds. At first the nuclei are in the center, but later most migrate to the periphery and form a single layer of cells comparable to the blastoderm of amphibian cells. A few nuclei remain in the central cavity to become yolk cells, and some at the posterior pole become separated into pole cells. [Pg.1898]

Strong electric fields and currents have been measured in a number of multicellular systems ranging from Fucus at the two cell state, a Cecropia moth oocyte-nurse cell cyncytum, amphibianand rodent limbs in regeneration and man (8, 22, 28, 3) Thus it is imperative to develop a formulation of multicellular phenomena. [Pg.184]

Immunofluorescent detection of TS protein was done with the use of monoclonal antibodies, developed by in vivo immunization of Balb/c mice with homogeneous recombinant rat hepatoma TS protein as an antigen. The specific anti-rat TS antibodies recognized also T. spiralis TS, as indicated by cross-reactivity on Western blot. Localization of the enzyme was based on analysis of pictures collected by confocal microscopy. Two types of T spiralis muscle larvae preparations were studied muscle larvae isolated from mouse muscles by a procedure destroying nurse cells and muscle larvae remaining in nurse cells, isolated as an intact nurse cell preparation. [Pg.334]

Localization of thymidylate synthase protein was followed in selected T. spiralis developmental forms, including (i) juvenile muscle larvae, (ii) infective muscle larvae (isolated either alone or in the intact nurse cell complex), (iii) adult worms and (iv) embryos developing within adult worms. [Pg.348]

The dytiscid beetles seem to represent an interesting intermediate between the typically panoistic and meroistic conditions. Morphologically they clearly belong to the meroistic type of ovariole development and nurse cells are actively engaged in the synthesis and transport of RNA into the oocytes. However, the oocyte nucleus also amplifies some of its nuclear DNA which results in the formation of a conspicuous Giardina s body (Urbani and Russo-Caia, 1964, 1969 Bier et al, 1967 Ficq and Urbani, 1969). [Pg.108]

Some isozymes that play an important role in embryogenesis are stored during oogenesis. It was suggested that in some animals these compounds are synthesized in the nurse cells and then transported into the oocyte (see previous chapter). Therefore, the beginning of individual development, or more exactly its conditional beginning, should be placed somewhere before the mature egg (Astaurov, 1974). [Pg.129]

A 52-year-old man was admitted to the hospital for abdominal surgery. He developed complications postoperatively and was intubated 6 days ago. The nurses note an increase in the amount and purulence of his sputum. Attempts yesterday and today to wean the patient off the ventilator have failed. He is sedated but does respond to commands. His temperature is 38.4°C, his blood pressure is 120/84 mm Hg, and his white blood cell (WBC) count is 14.2/mm3 with a cell differential of 76% neutrophils, 4% bands, 16% lymphocytes, and 4% monocytes. [Pg.1051]

Nurse In this final session, I wonder if it would be useful for us to identify what we think are the most important problems that ought to be addressed in the area of the cell cycle and development. [Pg.248]

The Carman-Kozeny equation relates the drop in pressure through a bed to the specific surface of the material and can therefore be used as a means of calculating S from measurements of the drop in pressure. This method is strictly only suitable for beds of uniformly packed particles and it is not a suitable method for measuring the size distribution of particles in the subsieve range. A convenient form of apparatus developed by Lea and Nurse 22 1 is shown diagrammatically in Figure 4.4. In this apparatus, air or another suitable gas flows through the bed contained in a cell (25 mm diameter, 87 mm deep), and the pressure drop is obtained from hi and the gas flowrate from h2. [Pg.203]

The lymph node microenvironment represents a niche where CLL cells interact with different types of cells including monocyte-derived nurse-like cells (NLC), CD3+ CD4+ CD154+ T cells, mesenchymal stromal cells, dendritic cells, and endothelial cells (15). In addition to cell-cell interactions, CLL cells are also exposed to a variety of soluble factors such as antigens, cytokines, and chemokines (2). It is the combination of such signals that renders CLL cells less susceptible to chemotherapy and promotes clonal evolution and drug resistance. Thus, the role of the microenvironment needs to be carefully considered in order to develop novel and more effective therapies for CLL treatment (16). In particular, the efficacy of new drugs must be evaluated under experimental conditions that recapitulate (or at least partially mimic) the CLL microenvironment. [Pg.218]


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See also in sourсe #XX -- [ Pg.299 ]




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