Nucleic structures, polymorphic

Keywords Berberine Palmatine Coralyne Polymorphic nucleic acid structures Alkaloid-nucleic acid interactions... 

This technique provides an easy and convenient method to evaluate the association of small molecules to various polymorphic forms of nucleic acid structures from the measurement of absorbance changes in the absorption maximum of the hgand, where the nucleic acid has no absorbance. Information about overall DNA/RNA base preference and nature of binding can also... 

Das S (2000) Biophysical studies on the interaction of benzophenanthridine alkaloids with polymorphic nucleic acid structures. PhD Thesis, Jadavpim University... 

Protein crystal structures are archived in the Protein Data Bank (PDB) (Bernstein et al. 1977 Berman et al. 2000). About 5 per cent of the approximately 14 000 (December 2000) entries ( 12 500 proteins, peptides, and viruses, 900 nucleic acids, 600 pro-tein/nucleic acid complexes, 20 carbohydrates) contain the qualifier form in the compound name/descriptor field, and most of those refer to polymorphic varieties. In biomolecular crystallography great efforts are expended varying crystallization conditions in the attempts to obtain single crystals suitable for structural investigations... 

Nielsen DA, Novoradovsky A, Goldman D (1995). SSCP primer design based on single-strand DNA structure predicted by a DNA folding program. Nucleic Acids Res., 23 2287-2291. Orita M, Suzuki Y, Sekiya T, Hayashi K (1989). Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics, 5 874-879. 

Hammond-Kosack, M. C., Dobrinski, B., Lurz, R., Docherty, K., Kilpatrick, M. W. (1992). The human insulin gene linked polymorphic region exhibits an altered DNA structure. Nucleic Acids Res 20, 231-236. 

Biomacromolecules were exploited as templates in the enzymatic synthesis of electronic and photonic materials for the development of biofunctional complexes and selective biosensors. The aniline was polymerized by the catalysis of HRP on a calf thymus DNA matrix consisting of a synthetic oligonucleotide (poly[dA-dC].poly[dG-dT]) [54]. The complexation of PANI with DNA and the ohgonucleotide was found to induce reversible changes in the secondary structure of the nucleic acid template, leading to the formation of an overwound polymorph. The melting behavior of the PANI/DNA complex demon-... 

The Institute of Biophysics developed into an internationally recognized center of bioelectrochemical research headed by professor E. Palecek. Electrochemical behavior of nucleic acids and proteins was studied systematically, and sensitive methods were developed for their determination. It was proved that DNA structure depends on the sequence of nucleotides [303, 304], or that the double heUx of DNA is polymorphous [305]. Activities of the Bmo group of bioelectrochemists were several times reviewed, e.g., [306]. Institute of Biophysics cooperated with Institute of Physical Chemistry of the Bmo University [307-309] (bioelectrochemistry of adenine, guanine, and guanidine). 

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