Nucleic acid polymorphism

Fig. 6. Nucleic acid polymorphs Fibrous polynucleotides unwinding right- to left-handed helices. Olson-and Z-families omitted. Modified from ref. 24.

Wittwer, C. T. Gundry, C. High resolution genotyping of nucleic acid polymorphisms using melting curve analysis. Eur. Pat. Appl. EP 1362928, 2003 Chem. Abstr. 2003,139, 376184. 

A2) In spite of the high individual frequencies, bond length and bond angle vibrations participate in quasi-classical low frequency collective normal modes. Bond angle bending is necessary for the flexibility of five-membered rings, which plays a key role in the polymorphism of nucleic acids. 

Keywords Berberine Palmatine Coralyne Polymorphic nucleic acid structures Alkaloid-nucleic acid interactions... 

This technique provides an easy and convenient method to evaluate the association of small molecules to various polymorphic forms of nucleic acid structures from the measurement of absorbance changes in the absorption maximum of the hgand, where the nucleic acid has no absorbance. Information about overall DNA/RNA base preference and nature of binding can also... 

Das S (2000) Biophysical studies on the interaction of benzophenanthridine alkaloids with polymorphic nucleic acid structures. PhD Thesis, Jadavpim University... 

Tautz, D. (1989) Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Research 17, 6463-6471. 

Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531-6535. 

Biomolecular MS and in particular MALDI-TOF-MS (see Sections 2.1.22 and 2.2.1) permit the routine analysis of oligonucleotides up to 70-mers, intact nucleic acids, and the direct detection of DNA products with no primer labels with an increase in analysis speed and mass accuracy especially in contrast to traditional DNA separation techniques such as slab gels or capillary electrophoresis. Applications focus on the characterization of single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs). Precise and accurate gene expression measurements show relative and absolute numbers of target molecules determined independently of the number of PCR cycles. DNA methylation can be studied quantitatively. 

Hameed AAA, Jean N, Stephen O, Andy PM (2008) Improved single nucleotide polymorphisms detection using conjugated polymer/surfactant system and peptide nucleic acid. Biosens Bioelectron 23 1466-1472... 

Blondal T, Waage BG, Smarason SV, Jonsson F, Fjalldal SB, et al. 2003. A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms. Nucleic Acids Res 15 el55. 

Guo, Z., Guilfoyle, R.A., Thiel, A.J., Wang, R., and Smith, L.M., Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acid Res., 22(24), 5456-5465, 1994. 

This chapter will review some of the key applications presented by protein microarrays. The use of profein microarrays sfems from works on gene expression arrays described earlier. However, rmlike ifs predecessors whose process formats (mutation detection, polymorphism screening, gene expression analysis, etc.) are essentially based upon solid-phase hybridization of nucleic acid complementary strands, the protein array may play different roles and comprise a variety of formafs. 

Wong KK, Tsang YT, Shen J et al. Allelic imbalance analysis by high-density single-nucleotide polymorphic allele (SNP) array with whole genome amplified DNA. Nucleic Acids Res 2004 32 e69. 

Hasegawa T, Kim HS, Fukushima M et al. Sequence analysis of the 5 -flanking regions of human dihydropyrimidine dehydrogenase gene identifieation of a new polymorphism related with effeets of 5-fluorouraeil. Nucleosides Nucleotides Nucleic Acids 2005 24 233-242. 

T.J. Huang, M. Liu, L.D. Knight, W.W. Grody, J.F. Miller and C.M. Ho, An electrochemical detection scheme for identification of single nucleotide polymorphisms using hairpin-forming probes, Nucleic Acids Res., 30 (2002)e55. 

Reich, Z., Ghirlando, R. and Minsky, A. (1991) Secondary conformational polymorphism of nucleic acids as a possible functional link between cellular parameters and DNA packaging processes. Biochemistry, 30, 7828-7836. 

---