Nucleic acids sequencers, automated

Nucleotides and nucleic acids are critical tools in the areas of gene expression, therapeutics, and diagnostics. However, there are certain challenges associated with their large-scale purification and subsequent characterization. While solid-state oligonucleotide synthesis is relatively simple and can be totally automated, intra- and intermolecular associations may occur involving shorter sequences that may hybridize with the desired full length... 

Nucleic acid structures and sequences primary and secondary structure of DNA fragments, translocation of genes between two chromosomes, detection of nucleic acid hybridization, formation of hairpin structures (see Box 9.4), interaction with drugs, DNA triple helix, DNA-protein interaction, automated DNA sequencing, etc. 

Yamakawa, H., and Ohara, O. (1997). A DNA cycle sequencing reaction that minimizes compressions on automated fluorescent sequencers. Nucleic Acid Res. 25(6), 1311-1312. 

Nucleic acid-based technologies Nucleic acid probe Polymerase chain reaction, DNA amplication 16S rRNA sequencing techniques automated riboprinting... 

Subsequently, the technologies for sequence determination of nucleic acids have been revolutionized (more than once and the revolution continues). The processes have been refined and automated. In laboratories doing serious sequence determination work, one finds batteries of automated, robotic machines grinding out long sequences of DNA routinely. Let s take a quick look at what has been gained so far. 

Ansorge, W., B. Sproat, J Stegemann, C. Schwager, and M. Zenke, Automated DNA sequencing ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Res, 1987.15(11) 4593-602. 

Kaiser, R.J., et al. (1989) Specific-primer-directed DNA sequencing using automated fluorescence detection. Nucleic Acids Res 17 6087-102. 

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