Sequencing automation

Bozzini, M., DeFranco, R.J., Guga, P.J., Mattaliano, R.J., and Boyd, V.L., (1993). C-terminal Sequencing Automation and Performance Assessment for the Alkylated Thiohydantoin Method. Presented at the Seventh Symposium of the Protein Society, San Diego, CA. 

The recent revolution in molecular biology has brought with it an increased demand for the efficient chemical synthesis of short DNA segments, called oligonucleotides. The problems of DNA synthesis are similar to those of protein synthesis (Section 26.10) but are more difficult because of the complexity of the nucleotide monomers. Each nucleotide has multiple reactive sites that must be selectively protected and deprotected at the proper times, and coupling of the four nucleotides must be carried out in the proper sequence. Automated DNA synthesizers are now available, however, that allow the fast and reliable synthesis of DNA segments up to 200 nucleotides in length. 

Wootton, J. C. (1994) Non-globular domains in protein sequences automated segmentation using complexity measures. Comput Chem. 18, 269-285. 

Sequencing Automation Large-Scale Sequencing Methods differ in several parameters such as the length of a sequence (length) that is rehably produced without error (raw error rate), the overall speed (raw base sequence per second), and cost. 

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