Automated Sequencing

Automatic sequencing with its numerous improvements is now routinely performed. A cycle requires an hour or less and the amount of peptide needed is well below the earlier applied 1 micromol. Yet, errors are not rare in the interpretation of results and the sequence of a peptide can be considered firmly established only if it is corroborated by a second, independent procedure. 

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DNA from a gene contains hundreds to thousands of nucleotide units for which the sequence is needed in order to interpret its code. Sequencing methods require only small amounts (5 (tg) of purified DNA, which can be produced by cloning. Automated sequencers are available that can daily sequence DNA containing hundreds of nucleotide units. 

Dahiyat, B.I., Mayo, S.L. De novo protein design fully automated sequence selection. Science 278 82-87,... 

PSA Model Development Tools in the CAFTA Workstation include an event Wee developer, fault tree editor, quantification tools, cutset editor, and automated sequence eiiiio ... 

Ewing B et al. Base-calling of automated sequencer traces using Phred I Accuracy assessment. Genome Res 1998 8 175-185. 

Automated sequencing systems, 72 510 Automatic Chemical Agent Detector and Alarm, 5 830-831 Automatic discharging suspended magnets, 75 438-439 Automatic dishwashing (ADW), 70 285 Automatic dishwashing detergents (ADDs), 70 274, 285... 

Aspec Automatic sample preparation system Asted automated sequence trace diazylate enricher 231/401 HPLC autosampling injector 232/401 automatic sample processor and injector ... 

Ewing, B., Hillier, L., Wendl, M. C., and Green, P. (1998). Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Res. 8, 175-185. 

After PCR, 1.0 pi PCR product is mixed with 8.5 pi Hi-Di formamide and 0.5 pi GS 500 ROX-Standard (Applied Biosystems). The mixtures are loaded on an appropriate automated sequencer. The sequence run is performed as described in the manual. 

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