Nucleic acid polymerase chain reaction

Myal Y., Blanchard A., Watson P., CoRRiN M., Shiu R., Iwasiow B. Detection of genetic point mutations by peptide nucleic acid-mediated polymerase chain reaction clamping using paraffin-embedded specimens. Anal. Biochem. 2000 285 169-172. 

Sotlar K, Escribano L, Landt O, et al One-step detection of c-kit point mutations using peptide nucleic acid-mediated polymerase chain reaction clamping and hybridization probes. Am J Pathol 2003 162 737-746. 

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

Two principal types of nucleic acid-based methods, nucleic acid hybridization and polymerase chain reaction (PCR), are commonly used for the rapid identification of bacteria. A few other nucleic acid-based methods will also be mentioned. 

Krawczak, M., Reiss, J., Schmidtke, J. and Rosier, U. (1989) Polymerase chain reaction replication errors and reliability of gene diagnosis. Nucleic Acids Research 17, 2197-2201. 

Murray, V. (1989) Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Research 17, 8889. 

Primeran oligonucleotide or pair of oligonucleotides used to direct an activity to a region of nucleic acid. With PCR (polymerase chain reaction), a pair of primers defines the area of the genome to be amplified. 

The viral load test quantifies viremia by measuring the amount of viral RNA. There are several methods used for determining the amount of HIV RNA reverse transcriptase-coupled polymerase chain reaction, branched DNA, and nucleic acid sequence-based assay. Each assay has its own lower limit of sensitivity, and results can vary from one assay method to the other therefore, it is recommended that the same assay method be used consistently within patients. 

The Polymerase Chain Reaction. In the past, a major drawback of hybridization assays was their need for relatively large amounts of sample DNA to compensate for their low sensitivity. This problem has been surmounted in recent years by the development of powerful enzymatic techniques that can exponentially replicate specific DNA sequences in the test tube. With these techniques it is now possible to analyze vanishingly small samples that initially contain fewer than 10 copies of the sequence of interest. The new methods take advantage of the chemical properties of nucleic acids and of highly specialized enzymes that can repair and replicate DNA in vitro. 

Nucleic Acid Quantitation Using the Competitive Polymerase Chain Reaction... 

Nucleic acid quantitation using the competitive polymerase chain reaction... 

Becker-Andre M, Hahlbrock K. 1989. Absolute quantification using the polymerase chain reaction (PCR). Nucleic Acid Res 17 9347-9446. 

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