Replicating DNA The Polymerase Chain Reaction

Before or after reading the following sections on sequencing, go to www. pbs.org/wgbh/nova/genome/sequener. html and read Sequence for Yourself. 

Then the original template was TAACG. Now we will describe how this is done. 

DNA has a unique property. It is denatured by heating (much as proteins are), but it does so by separation of the two strands. This occurs if we heat DNA in solution 

Nuclease Large DNA fragments Insert in Bacterial 100-300 kb BAG vector replication  

Large DNA Nuclease Small DNA fragments Plasmid Billions of copies 

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