Oligonucleotide using

This group was effective in the synthesis of oligonucleotides using the phospho-triester approach. 

It was concluded that the crosslinking reaction proceeded through initial alkylation of N-7 of adenine by the aziridine ring, followed by guanine N-7 alkylation by the epoxide (Figure 11.12). This seems inconsistent with Armstrong s earlier conclusions, but the very short oligonucleotides used by Saito et al. may influence the DNA alkylation chemistry. 

With respect to template-directed syntheses, the syntheses of oligonucleotide using polynucleotide have already been attempted62-69. However, it has not necessarily been successful yet, since the binding constant of the nucleotide monomer with polynucleotide was not large, particularly in aqueous media. 

An early review on the template-directed synthesis of oligonucleotides using imidazo-lides of nucleoside S -phosphate is included in reference 153]. 

Primeran oligonucleotide or pair of oligonucleotides used to direct an activity to a region of nucleic acid. With PCR (polymerase chain reaction), a pair of primers defines the area of the genome to be amplified. 

Prepare an amine-modified oligonucleotide using any of the methods of Sections 1 or 2.1 (this chapter). Dissolve or buffer exchange 70 pg of the oligo into 25 pi of 0.1M sodium... 

It s also possible to select your DNA before you put it in the vector. If you know the sequence (or even part of it), DNA pieces (from genomic DNA or cDNA) with this sequence can be purified on a gel and identified by hybridization to an oligonucleotide using a Southern blot. Alternatively, if you know the sequence of the ends of your DNA, you can amplify it specifically by the polymerase chain reaction. There are lots of clever ways to find your DNA. 

E. Nordhoff, M. Schurenberg, G. Thiele, C. Lubbert, K.-D. Kloeppel, D. Theiss, H. Lehrach, and J. Gobom. Sample Preparation Protocols for MALDI-MS of Peptides and Oligonucleotides Using Prestructured Sample Supports. Int. J. Mass Spectrom., 226(2003) 163-180. 

Wu, K.J. Steding, A. Becker, C.H. Matrix-Assisted Laser Desorption-TOF-MS of Oligonucleotides Using 3-Hydroxy-picolinic Acid As an Ultraviolet-Sensitive Matrix. Rapid Commun. Mass Spectrom. 1993, 7,142-146. 

De Beilis, G. Salani, G. Battaglia, C. Pietta, P. Rosti, E. Mauri, P. ESl-MS of Synthetic Oligonucleotides Using 2-Propanol and Spermidine. Rapid Commun. Mass Spectrom. 2000,14, 243-249. 

Sonoda, R., Nishi, H., and Noda, K. (1998). Capillary gel electrophoresis of oligonucleotides using polymer solutions. Chromatographia 48, 569-575. 

The phosphoramidite of 8-Ph-dG has been prepared and used to site-specifically incorporate it into oligonucleotides using solid-phase DNA synthesis. In the 12-mer 5 -d(GCGCCXGCGGTG), where X = 8-Ph-dG, the adduct behaved very similar to 8-Me-dG with of the duplex being 5.6°C lower than the normal dG dC base pair, while the of 8-Ph-dG dG was 2.1°C higher than that of the mismatched dG dC base pair. This data suggested that, similar to 8-Me-dG, 8-Ph-dG exists primarily in the anti conformation when paired with dC, while the syn conformation may pair with dGMP. ... 

E. Hedberg, B. Langstrom, F-Labeling of oligonucleotides using succinimido 4-[ F] fluorobenzoate, Acta Chem. Scand. 52 (1998) 1034-1039. 

Directed Evolution to Increase the Substrate Range of Benzoylformate Decarboxylase 301 Table 2.2.1.1 Oligonucleotides used in this study. ... 

Each amplicon is amplified by PCR using the appropriate thermal cycling conditions [59, 71]. The amplicons are then purified from the dNTPs and the oligonucleotides using the microspin S-300 spin columns. 

Table 16.5 Enthalpy and melting temperature results from calorimetric studies of oligonucleotides used to generate data for nearest-neighbor base-stacking interactions. The poly designates a strand of base-pairs of undetermined length.

Figure 16.17 The cisplatin-bound synthetic oligonucleotide used in the Breslauer study.

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