Nucleic acids metal complexes

The bottom-up synthesis of metallic nanowires was further applied to construct a nanotransistor device.93 The sequence-specific winding of the homologous nucleic acid carried by the RecA-protein into the duplex DNA was used to address the nucleic acid/protein complex on the DNA scaffold (Fig. 12.27). The subsequent association of the anti-RecA antibody to the protein DNA complex, followed by the association of the biotinylated antiantibody, and the linkage of streptavidin-modified carbon nanotube deposited the tubes in the specific domain of the DNA scaffold. The further... 

It is well known that a great variety of biomolecules exist where metals and metalloids are bound to proteins and peptides, coordinated by nucleic acids or complexed by polysaccharides and small organic ligands such as organic acids.55 Most proteins contain amino acids with covalently bonded heteroelements such as sulphur, selenium, phosphorus or iodine.51 Several reviews have been published on the development of mass spectrometric techniques for bioanalysis in metal-lomics , which integrate work on metalloproteins, metalloenzymes and other metal containing biomolecules.1 51 53 54 56-59 The authors consider trace metals, metalloids, P and S (so-called... 

The ability to calculate redox potentials has powerfid implications. For example, elecfrocatalytic reactions of metal complexes, such as Ru(bpy)3 +, with DNA nucleobases, such as guanine, provide a sensitive method for detection of nucleic acids on surfaces in electrochemical DNA chips (see Nucleic Acid-Metal Ion Interactions) In these reactions, voltammetric oxidation of Ru(bpy)3 + to Ru(bpy)3 + in the presence of guanine produces an enhancement in the oxidative current due to the reaction of Ru(III) with guanine (Figure 2). On surfaces, the extent of elecfrocatalytic enhancement is indicative of the quantity of bound nucleic acid levels of DNA as low as 40 attomoles have been detected using related methods. ... 

Carbene Complexes Carbonyl Complexes ofthe Transition Metals Cyanide Complexes of the Transition Metals Dinuclear Organometallic Cluster Complexes Electron Transfer in Coordination Compounds Electron Transfer Reactions Theory Electronic Structure of Organometallic Compounds Luminescence Nucleic Acid-Metal Ion Interactions Photochemistry of Transition Metal Complexes Photochemistry of Transition Metal Complexes Theory Polynuclear Organometallic Cluster Complexes. 

Various metals have been complexed to oligonucleotides to produce hydrolytic or oxidative cleavage of a target nucleic acid. Lanthanide complexes covalently attached to oligonucleotides are known to cleave single-stranded... 

Apart from the understanding of the mechanism of anti-tumour action of cis-[PtCl2(NH3)2l a major interest in the area of nucleic acid-metal ion interactions is the possibility of sequence determination, using specific binding of metal complexes. 

In addition to serving structural and modulating roles in proteins which bind nucleic acids, metal ions also appear to be essential to the functioning of various complex enzymes that act on nucleic acids. At this stage our understanding of the participation of the metal ion in the catalytic chemistry of these enzymes is somewhat sketchy, and we are relying more on our current understanding of the... 

The ability to calculate redox potentials has powerful implications. For example, electrocatalytic reactions of metal complexes, such as Ru(bpy)3 +, with DNA nucleobases, such as guanine, provide a sensitive method for detection of nucleic acids on surfaces in electrochemical DNA chips see Nucleic Acid-Metal Ion Interaction In these reactions. 

Metal Ions can produce a large variety of other effects on nucleic acids that could be deleterious if they occur during genetic information transfer. Metal ions can bring about the degradation of RNA (17, 18, 19), changes in the specificity of enzymes that act on DNA (20), changes in the conformation of polynucleotides and nucleic acid - protein complexes (21). 

Although many studies on DNA and DNA-protein interaction have utilized metal shadowing to produce bright-held image contrast, dark-held TEM (annular or spectroscopic) of unstained or uranyl acetate-stained and metal-shadowed nucleic acid-protein complexes also provides useful resolution. In Figure 5, a short length of DNA (2356 base pairs), with attached progesterone receptor, is revealed by dark-held TEM. The RecA protein has the ability to... 

Two aspects of nanoparticles immobilization on biopolymers should be mentioned. First, most of the heavy metals bind simultaneously to several protein macroligands with a specific spatial environment and conformation. Triple complexes may also form composed of protein/metal/nucleic acid (metal = Au, Pt, Pd). Perhaps the presence of these structures provides the anticarcinogenic effect achieved on introduction of these metals into organisms. Second, heavy metals, such as Au(T), react with native Zn-, Cd-, and Co-thioneins by replacing selectively Zn and even Cd when added in excess. This process is characterized by only minimal changes in the protein conformation. 

Hsiao C, Tannenbaum E, VanDaeusen H, Hershkovitz E, Pemg G, Tannenbaum AR, Williams LD. Complexes of nucleic acids with group I and II cations. In HudNV, Editor. Nucleic acid-metal ion interactions. Cambridge Royal Society of Chemistry 2009. pp 1-38. Permyakov EA. Metalloproteins. New Jersey Wiley 1009. 

Technetium-99m coordination compounds are used very widely as noniavasive imaging tools (35) (see Imaging technology Radioactive tracers). Different coordination species concentrate ia different organs. Several of the [Tc O(chelate)2] types have been used. In fact, the large majority of nuclear medicine scans ia the United States are of technetium-99m complexes. Moreover, chiral transition-metal complexes have been used to probe nucleic acid stmcture (see Nucleic acids). For example, the two chiral isomers of tris(1,10-phenanthroline)mthenium (IT) [24162-09-2] (14) iateract differentiy with DNA. These compounds are enantioselective and provide an addition tool for DNA stmctural iaterpretation (36). 

Nucleic acid recognition by metal complexes of bleomycin 99CRV2797. 

Photocleavage of nucleic acids with participation of metal complexes with heterocyclic ligands 98CRV1171. 

The stereochemistry of metal complexes of nucleic acid constituents. D. J. Hodgson, Prog. Inorg. Chem., 1977, 23,211-254 (175). 

Metal complexes of nucleic acid derivatives and nucleotides binding sites and structures. L. G. Marzilli, Adv. Inorg. Biochem., 1981, 3, 48-85 (138). 

Stereoselectivity in the binding of transition metal chelate complexes to nucleic acid constituents bonding and non-bonding effects. L. G. Marzilli and T. J. Kistenmacher, Acc. Chem. Res., 1977, 10,146-152 (29). 

Moucheron C, Kirsch-De Mesmaeker A, Kelly JM (1998) Photophysics and Photochemistry of Metal Polypyridyl and Related Complexes with Nucleic Acids. 92 163-216... 

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