Nucleic acids anion-exchange chromatography

Lipoteichoic acids (from gram-positive bacteria) [56411-57-5J. Extracted by hot phenol/water from disrupted cells. Nucleic acids that were also extracted were removed by treatment with nucleases. Nucleic resistant acids, proteins, polysaccharides and teichoic acids were separated from lipoteichoic acids by anion-exchange chromatography on DEAE-Sephacel or by hydrophobic interaction on octyl-Sepharose [Fischer et al. Ear J Biochem 133 523 1983]. 

Drager, R. R. and Regnier, F. E., Application of the stoichiometric displacement model of retention to anion-exchange chromatography of nucleic acids,... 

The ionizable phosphate group present in organic phosphorus species provides the basis for separation by anion exchange chromatography. This approach has been applied to the separation of phosphate, inositol phosphates, organic condensed phosphates, nucleic acids, nucleoside phosphonates, phospholipids and other organic phosphorus species. 

Anion-exchange chromatography on manbranes, disks, and rods bearing mainly quaternary amino groups or DEAE groups as ligands has been used for the separation of serum proteins, microbial proteins and enzymes, membrane proteins, cytokines, or nucleic acids [40,69,249-254]. BSA and HSA, a-chymotrypsinogen, lysozyme, trypsin inhibitor, cytochrome c, ovalbumin, a-lactalbumin, conalbumin. 

Several functional groups have been used to obtain cellulose anion exchangers [aminoethyl (AE), diethyl-aminoethyl (DEAE)], or cation exchangers [car-boxymethyl (CM), phosphate (P)] for thin-layer chromatography. PEI cellulose is not a chemically modified cellulose, but a complex of cellulose with polyethyleneimine. These cellulose exchangers are particularly useful for the separation of proteins, aminoacids, enzymes, nucleobases, nucleosides, nucleotides, and nucleic acids. 

Ion-exchange chromatography has many clinical applications, including the separation of amino acids, peptides, proteins, nucleotides, oligonucleotides, and nucleic acids. Another important application of ion-exchange chromatography is the separation and removal of inorganic ions from aqueous mixtures. Thus most water purification units used to prepare deionized water for the laboratory contain "mixed-bed columns of cation and anion resins (see Chapter 1). 

Use As an anion exchanger in chromatography. It is less basic than DEAE-cellulose and serves to separate viruses, nucleic acids, and nucleoproteins. 

Once the bacterial cells have been lysed and a crude preparation of plasmid has been obtained, several other methods are available for purification of plasmid from the crude nucleic acid preparation, such as cesium chloride ultracentrifugation, gel filtration/size exclusion chromatography, anion exchange (used in the Qiagen kit mentioned above), and HPLC (Table 2) (see Note 2). 

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