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Gel filtration size exclusion

Purify the derivatized dendrimer using gel filtration (size exclusion chromatography) on a desalting column or through use of ultrafiltration spin-tubes (for G-4 and above). For smaller dendrimers, the derivatives may be purified by repeated precipitation from a meth-anolic solution by addition of ethyl acetate, dioxane, or benzene. The SPDP-dendrimer may be dried by lyophilization (if in water or buffer) or by solvent evaporation in vacuo (if the precipitation method was used). [Pg.358]

Molecular exclusion chromatography, also known as gel filtration, size exclusion chromatography, gel permeation chromatography, or simply gel chromatography, is another separation method based on differences in molecular size. [Pg.307]

Gel filtration/size exclusion Size Desalting, large molecules... [Pg.230]

Once the bacterial cells have been lysed and a crude preparation of plasmid has been obtained, several other methods are available for purification of plasmid from the crude nucleic acid preparation, such as cesium chloride ultracentrifugation, gel filtration/size exclusion chromatography, anion exchange (used in the Qiagen kit mentioned above), and HPLC (Table 2) (see Note 2). [Pg.264]

Fractionation, Caprylic acid, Gel filtration, Size-exclusion chromatography, Fast Protein Liquid... [Pg.15]

Gel filtration (size exclusion) Gel filtration chromatography (also called size exclusion chromatography) employs porous beads with a defined pore size distribution as the stationary phase. Small molecules can enter the entire intraparticular pore space and hence elute last, whereas large molecules are excluded from all pores and hence elute first. Molecules of intermediate size are found that can enter a certain... [Pg.2690]

Gel filtration Size exclusion chromatography performed with aqueous solvents for the separation of biopolymers. [Pg.63]


See other pages where Gel filtration size exclusion is mentioned: [Pg.39]    [Pg.67]    [Pg.361]    [Pg.236]    [Pg.83]    [Pg.1333]    [Pg.137]    [Pg.333]    [Pg.145]    [Pg.145]    [Pg.146]    [Pg.3717]    [Pg.62]    [Pg.62]    [Pg.121]    [Pg.303]    [Pg.385]    [Pg.387]    [Pg.389]    [Pg.393]    [Pg.395]    [Pg.937]   


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