Nramp

Nramp Natural resistance associated macrophage proteins ... 

Portnoy, M. E., Liu, X. F. and Culotta, V. C. (2000). Saccharomyces cerevisiae expresses three functionally distinct homologues of the Nramp family of metal transporters, Mol. Cell Biol., 20, 7893-7902. 

Kehres, D. G., Zaharik, M. L., Finlay, B. B. and Maguire, M. E. (2000). The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen, Mol. Microbiol., 36, 1085-1100. 

Patzer, S. I. and Hantke, K. (2001). Dual repression by Fe2+-Fur and Mn2+-MntR of the mntH gene, encoding an NRAMP-like Mn2+ transporter in Escherichia coli, J. Bacteriol., 183, 4806—4813. 

Thomine, S., Wang, R., Ward, J. M., Crarvford, N. M., and Schroeder, J. I., 2000, Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes, Proc. Natl. Acad. Sci. U.S.A. 97 4991-4996. 

The stability of the reagent to the assay conditions should also be ascertained. The instability of azides and diazo compounds towards thiols was mentioned earlier, and it would be prudent to ensure that all new reagents whether they contain familiar photoactivatable groups or not are stable to the prevailing chemical and enzymatic conditions. In an attempt to label the cAMP receptor of D. discoideum, Wallace and Frazier (1979b) found that 8-N rcAMP was converted to NrAMP by the phosphodiesterase of a crude membrane preparation. The NrAMP specifically labeled actin and not the cAMP receptor. 

Nevo Y, Nelson N. The NRAMP family of metal-ion transporters. Biochim. Biophys. Acta 2006 1763 609-620. 

Seefeldt, L. C., Hoffman, B. M., Dean, D. R. (2009). Mechanism of Mo-dependent nitrogenase. The Annual Review ofBiochemistry, 78,701—722. Ueki, T, Furano, N., Michibata, H. (2011). A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea Biochimica et Biophysica Acta, 1810, 457—464. 

Agranoff, D., I.M. Monahan, J.A. Mangan, P.D. Butcher and S. Krishna. Mycobacterium tuberculosis expresses a novel pH-dependent divalent cation transporter belonging to the Nramp family. J. Exp. Med. 

Chen, H., G.C. Waldbieser, C.D. Rice, B. Elibol and L.A. Hanson. Isolation and characterization of a channel catfish natural resistance associated macrophage protein (Nramp) gene. Dev. Comp. Immunol. 26 517-531, 2002. 

Dorschner, M.O. and R.B. Phillips. Comparative analysis of two Nramp loci from rainbow trout. DNA Cell Biol. 18 573-583, 1999. 

Saeij, J.P., G.F. Wiegertjes and R.J.M. Stet. Identification and characterization of a fish natural resistance-associated macrophage protein (Nramp) cDNA. Immunogenetics 50 60-66, 1999. 

SLCll (3) Proton-coupled divalent metal ion NRAMP, DCT Ubiquitous... 

Cadmium, again with a much higher affinity to thiol compounds, is a toxic-only element. Only one case of Cd(II) as a trace element has been reported in the absence of Zn(II), Cd(II) was used by some diatoms in the active site of carbonic anhydrase (Lane and Morel 2000). Cd(II) enters the cell by (CorA- and NRAMP-like) uptake systems (Nies 1999), binds to thiol compounds (thereby exerting toxicity), and is then reexported by efflux systems (P-, CBA, or CDF type) (Nies 2003). 

Picard V, Govoni G, Jabado N and Geos P (2000) Nramp 2 (DCTl/DMTl) expressed at the plasma membrane transports iron and other divalent cations... 

A number of genes involved in metal transport in plants have been identified. Many of these belong to previously described transporter families such as the P-type APTases (Axelsen et al. 1998) and the Nramp proteins (natural resistance-associated macrophage protein) (Cellier et al. 

Rodrigues V, Chia Wand Geos P (1995) Nramp defines a family of membrane proteins. Proc Natl Acad Sci USA 92 10089-10093. 

Curie C, Alonso JM, Lejean M, Ecker J-R and BeiatJ-F (2000) Involvement of Nramp-1 from Arabidopsis thaliana in iron transport. Biochem J 347 3749-3755. 

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