Nipkow disk

The TSM can be modified for reflected light confocal microscopy (TSRLM). In that case, one Nipkow disk above the objective serves both for the illuminating beams and the reflected image-forming rays. 

Grant, D. M., Elson, D. S., Schimpf, D., Dunsby, C., Requejo-Isidro, J., Auksorius, E., Munro, I., Neil, M. A. A., French, P. M. W. Nye, E., Stamp, G. and Courtney, P. (2005). Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source. Opt. Lett. 30, 3353-5. 

Ni-P adhesion, 9 705. See also Nickel phosphorus entries Ni-P alloys, solderability, 9 707, 708 NIPAm hydrogels, 13 738 Ni-P density, 9 705 Ni-P electrical resistivity, 9 706 Ni-P ferromagnetic properties, 9 706 Nipkow disk, 16 484 Ni-P mechanical properties, 9 706 Niranium N/N, base-metal dental alloy, 8 309t... 

Opera Perkin Elmer Laser confocal with Nipkow disk Available... 

Pathway 435 BD Biosciences Wide field with Nipkow disk No... 

Masters BR, Kino GS. Charge coupled devices for quantitative Nipkow Disk real-time scanning confocal microscopy. In Shotton D, ed. Electron light microscopy The principles and practice of video-enhanced contrast, digital intensified fluorescence, and confocal scanning light microscopy. New York Wiley-Liss, 1993. 

Kino, G. S. (1989). Intermediate optics in Nipkow disk microscopes. In The Handbook of Biological Confocal Microscopy (J. Pawley, ed.), 1st ed., pp 105-126. IMR Press, Madison, WI. Matsumoto, B. (1993). Cell biological applications of confocal microscopy. Methods Cell Biol. 38, 1-380. 

Finally, it is not necessary to move the object to generate an image. Instead, the source may be scanned so that it illuminates the object one point at a time. The image of the source may be scanned optically, as with a pair of mirrors, but a simpler system based on a Nipkow disk has more recently been developed. In such a system, a plate (the Nipkow disk) that contains a series of holes is rotated at high speed, and each hole scans one line in the object. The image may be observed by eye or by a video camera, or it may be digitized with a computer. 

In 1968, M. Petran and his collaborators applied the Nipkow disk principle to develop a tandem scanning reflected light microscope in which the single... 

The GARY module (ATTO Bioscience) contains a Nipkow disk with pinholes and can be added to a variety of widefield microscopes. The Olympus Disk Scanning Unit (DSU) can be fitted to Olympus microscopes and uses a rotating disk with a pattern of shts instead of pinholes. Its optical sectioning performance can be altered by the insertion of disks with different sht widths. 

Another confocal scanning method uses a rapidly rotating disk containing many holes in the confocal plane (a Nipkow disk). These holes act as confocal apertures and mechani-... 

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