NIH/3T3 fibroblasts

Cunningham, C.C., Stossel, T.P., Kwiatowski, D.J. (1991). Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science, 251, 1233-1236. 

NGF Nerve growth factor NGPS Normal guinea-p serum NIH 3T3 (fibroblasts) National Institute of Health 3T3-Swiss albino mouse fibroblast NIMA Non-inherited maternal antigens... 

Kiss, Z., and Deh, E., 1995, Preferenhal inhibition of phorbol ester-induced hydrolysis of phosphatidylethanolamine by N-acetylsphingosine in NIH 3T3 fibroblasts. FEES Lett. 

Ferry DR, Smith A, Malkhandi J, F e DW, deTakats PG, Anderson D, Baker J, Kerr DJ (1996) Phase 1 clinical trial of the flavonoid quercetin pharmacokinetics and evidence for in vivo tyrosine kinase inhibition. Clin Cancer Res 2 659-668 Findik D, Song Q, Hidaka H, Lavin M (1995) Protein kinase A inhibitors enhance radiation-induced apoptosis. J Cell Biochem 57 12-21 Fine RL, Patel J, Chabner BA (1988) Phorbol esters induce multidrug resistance in human breast cancer cells. Proc Natl Acad Sci USA, 85 582-586 Finkenzeller G, Marme D, Hug H (1992) Inducible overexpression of human protein kinase C in NIH 3T3 fibroblasts results in growth abnormalities. Cell Signall 4 163-177... 

Persons DA, Wilkinson,WO, Bell RM, Finn OJ (1988) Altered growth regulation and enhanced tumorigenicity of NIH 3T3 fibroblasts transfected with protein kinase C-I cDNA. Cell 52 447-458... 

Other assays are being explored in HTS platforms to identify potential inhibitors of invasion, for example, compounds that affect the shape or morphology of cells or their ability to generate invadopodia. Quintavalle et al. recently described such a method initially using Src-transformed NIH 3T3 fibroblasts grown on 384-well optical plates. Imaging of cellular and nuclear morphology combined with phalloidin-stained F-actin was used to discriminate compounds which reduced (or enhanced) the number of... 

Ahn, H.Y., Hadizadeh, K.R., Seul, C., Yun, Y.P., Vetter, H., and Sachinidis, A., Epigallocathe-chin-3-gallate selectively Inhibits the PDGF-BB-induced intracellular signaling transduction pathway in vascular smooth muscle cells and inhibits transformation of ai-transfected NIH 3T3 fibroblasts and human glioblastoma cells (A172), Mol. Biol. Cell, 10, 1093, 1999. 

Zhao Q, Chen N, Wang W-M, Lu J, Dai B-B. Effect of transforming growth factor-beta on activity of connective tissue growth factor gene promoter in mouse NIH/3T3 fibroblasts. Acta Pharmacologica Sinica 2004, 25, 485 189. 

Murine NIH 3T3 fibroblasts, C2C12 myoblasts, rat aorta smooth muscle cells, and rat aortic endothelial cells Stencilguided plasmainduced oxidation of proteinresistant polymer network 2003 [96]... 

GRGDS Methoxy-capped PEG NIH-3T3 fibroblasts Local modification of NHS-terminated PEG with 2-methoxyethylamine through a micropatterned photoresist (photolithography), backfill with peptide after resist removal 2008 [157]... 

RODS Poly(A-isopropylacrylamide) (PIPAAm) NIH-3T3 fibroblasts Electron beam activated polymerization of 2-carboxy-iV-isopropylacrylamide through a mask on PIPAAm (or vice versa), subsequent NHS mediated binding of peptide to carboxyl functional groups 2007 [173]... 

Like the other 5-Hrl receptors, the major signaling pathway of the 5-HT1D receptor is to the inhibition of AC through pertussis toxin-sensitive G proteins in the substantia nigra (153), in MDCK cells (154), and in transfected C6 glioma and NIH-3T3 fibroblast cells (116,134,155,156). 

Varrault A, Joumot L, Audigier Y, Bockaert J. Transfection of human 5-hydroxy-tryptamine1A receptors in NIH-3T3 fibroblasts effects of increasing receptor density on the coupling of 5-hydroxytryptamine1A receptors to adenylyl cyclase. Mol Pharmacol 1992 41 999-1007. 

Fig. 3.11 (a) Averaged Raman spectrum of 50 different NIH/3T3 fibroblast cells measured at a 785 nm excitation wavelength, 50 mW laser power at the sample for 180 s. (b-e) SER spectra taken by excitation of NS in different cells at the same wavelength with 5 s acquisition time and 3 mW laser power at the sample [56]... 

Fig. 3.12 (a) TEM image taken of a single NS in NIH/3T3 fibroblast cell located near the nuclear membrane, (b) SERS spectra of 4 mercaptobenzoic-acid functionalized NS in NIH/3T3 fibroblast. Overlying spectra depict NS-4MBA in solution black) and inside NIH/3T3 cells red) determining the pH to be 7.4 and 6.5, respectively [56]... 

Chen M et al (2007) Role of fiber diameter in adhesion and proliferation of NIH 3T3 fibroblast on electrospun polycaprolactone scaffolds. Tissue Eng 13(3) 579-587... 

In a third example of this methodology Mei et al. [65] produced a polymer containing the fibronectin-based peptide sequence GRGDS, a well-known cell adhesion sequence. This time, however, the polymerization of hydroxyethyl methacrylate was carried out on the sohd support in methyl ethyl ke-tone/propanol (7 3), resulting in a bio-hybrid with a polydispersity of 1.5. This peptide-polymer hybrid material was used as a support for the growth of mouse NIH-3T3 fibroblasts. It was shown that the cells adhered better to the polymer containing the peptide than to the unfunctionalized control polymer. This highhghts one of the many potential applications for protein-polymer hybrid materials. 

Stable transfection in NIH-3T3 fibroblasts yielded specific binding with I-LSD, weak affinity for spiperone and high affinity for the mixed 5-HT2a/5-HT2c... 

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