Human glioblastoma

GaUi R, Binda E, OrfaneUi U, CipeUetti B, Gritti A, De Vitis S, Eiocco R, Foroni C, Dimeco F Vescovi A (2004) Isolation and characterization of tumorigenic, stem-hke neural precursors from human glioblastoma. Cancer Res 64 7011-7021... 

Rempel SA, Dudas S, Ge S, Gutierrez JA (2000) Identification and localization of the cytokine SDFl and its receptor, CXC chemokine receptor 4, to regions of necrosis and angiogenesis in human glioblastoma. Chn Cancer Res 6 102-111 Reya T, Morrison SJ, Clarke ME, Weissman IL (2001) Stem cells, cancer, and cancer stem cells. Nature 414 105-111... 

Barbero S, Bonavia R, Bajetto A, et al. Stromal cell-derived factor la stimulates human glioblastoma cell growth through the activation of both extracellular signal-regulated kinases 1/2 and AKT. Cancer Res 2003 63 1969-1974. 

P. Manning, C.J. McNeil, J.M. Cooper, and E.W. Hillhouse, Direct, real-time sensing of free radical production by activated human glioblastoma cells. Free Radical Bio. Med. 24, 1304-1309 (1998). 

S. K. Hobbs, G. Shi, R. Homer, G. Harsh, S. W. Atlas and M. D. Bednarski, Magnetic resonance image-guided proteomics of human glioblastoma multiforme, J. Magn. Reson. 

Kraus, A., et ah. Expression of alternatively spliced mdm2 transcripts correlates with stabilized wild-type p53 protein in human glioblastoma cells. IntJ Cancer, 1999, 80(6), 930-4. 

In mouse cerebellum and cerebral cortex by IHC and WB (Zehnder et al, 2001). Gene amplification for 25-hydroxyvitamin D(3) 1,alpha-hydroxylase and nonfunctional mRNA splice variants in human glioblastoma multiforme (Maas et al, 2001 Diesel et al, 2004, 2005). 

Aromatase immunoreactivity in rat, human glioblastoma (Yague et al., 2004). Human fetal brain and intestine had more CYP19 mRNA than other tissues (Price et al., 1992). 

RT-PCR for CYP1A2 is negative in a series of 22 human glioblastomas and 4 anaplastic astrocytomas (Kirches et al., 1999). 

Diesel B, Radermacher J, Bureik M, Bernhardt R, Seifert M, et al. 2005. VitaminD(3) metabolism in human glioblastoma multiforme functionality of CYP27B1 splice variants, metabolism of calcidiol, and effect of calcitriol. Clin Cancer Res 11 5370-5380. 

Huang etal. (2002) prepared an antibody array for the simultaneous detection of 43 cytokines. They were able to verify the down-regulation of MCP-1 cytokine in transfected cells (human glioblastoma cells transfected with cx43 expression vector) relative to control cells. The antibody array is an emerging technology. In at least one study based upon the use of a commercial membrane format, the cytokine microarray failed to accurately determine cytokine levels in bacterial and lipopolysaccharide (LPS)-stimu-lated whole human blood (Copeland, 2004). 

Taken together, the various observations suggest that FBXLIO has context-dependent pro- and anti-oncogenic activities. In line with this, FBXLIO expression was reported to be significantly decreased in human glioblastomas relative to normal brain tissue [54], while FBXLIO was found to be overexpressed in lymphomas and mammary adenocarcinomas [56]. 

Ahmad S, Mineta T, Martuza RL, Glazer RI (1994) Antisense expression of protein kinase C alpha inhibits the growth and tumorigenictity of human glioblastoma cells. Neurosurgery 35 904-908... 

Kondo S, Barnett GH, Kara H, Morimura T, Takeuchi J (1995a) MDM2 protein confers the resistance of a human glioblastoma cell line to cisplatin-induced apoptosis. Oncogene 10 2001-2006... 

Xiao H, Goldthwait DA, Mapstone T (1994) The identification of four protein kinase C isoforms in human glioblastoma cell lines PKC alpha, gamma, epsilon, and zeta. J Neurosurg 81 734-740... 

Feedback circuit among INK4 tumor suppressors constrains human glioblastoma development. Cancer Cell 13 355-364... 

Mazzoleni S, Politi LS, Pala M et al (2010) Epidermal growth factor receptor expression identifies functionally and molecularly distinct tumor-initiating cells in human glioblastoma multiforme and is required for gliomagenesis. Cancer Res 70 7500-7513... 

Ahn, H.Y., Hadizadeh, K.R., Seul, C., Yun, Y.P., Vetter, H., and Sachinidis, A., Epigallocathe-chin-3-gallate selectively Inhibits the PDGF-BB-induced intracellular signaling transduction pathway in vascular smooth muscle cells and inhibits transformation of ai-transfected NIH 3T3 fibroblasts and human glioblastoma cells (A172), Mol. Biol. Cell, 10, 1093, 1999. 

Chan JA, Krichevsky AM, Kosik KS. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 2005 65 6029-6033. 

Frederick L, Wang XY, Eley G et al. Diversity and frequency of epidermal growth factor receptor mutations in human glioblastomas. Cancer Res 2000 60 1383-1387. 

Dhandapani KM, Mahesh VB, Brann DW. 2007. Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NFkappaB transcription factors. J Neurochem 102 522-538. 

Karmakar S, Banik NL, Patel SJ, Ray SK. 2006. Curcumin activated both receptor-mediated and mitochondria-mediated proteolytic pathways for apoptosis in human glioblastoma T98G cells. Neurosci Lett 407 53-58. 

Wolburg, H., et al. 2003. Localisation of claudin-3 in tight junctions of the blood-brain barrier is selectively lost during experimental autoimmune encephalomyelitis and human glioblastoma multiforme. Acta Neuropathol 105 586. 

L. Maletinska, E.A. Blakely, K.A. Bjomstad, D.F. Deen, L.J. Knoff and T.M. Forte, Human glioblastoma cell lines levels of low-density lipoprotein receptor and low-density lipoprotein receptor-related protein, Cancer Res. 60 (2000) 2300-2303. 

G. Bu, E.A. Maksymovitch, H. Geuze and A.L. Schwartz, Sub-cellular localization and endocytic function of low density lipoprotein receptor-related protein in human glioblastoma cells,... 

TFP (5) was found to be a very effective blocker of human Kv2.1 potassium channels expressed in human glioblastoma cells [253]. As for other types of channels, for Kv2.1 the TFP-induced block was also dose-dependent (IC50 = 1.21 xM). Some of the other, non-phenothiazine drugs were even more effective blockers for the most potent, fluspirilene (an antipsychotic agent), substantial block was observed at 30 nM. 

Sugawa, N., Ekstrand, A. J., James, C. D., and Collins, V. P. (1990). Identical splicing of aberrant epidermal growth factor receptor transcripts from amplified rearranged genes in human glioblastomas. Proc. Natl. Acad. Sci. U.S.A. 87, 8602-8606. 

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