Nicotinamide 1-oxide

In a 2-1. round-bottomed flask with ground-glass joint (Note 1) are placed 100 g. (0.82 mole) of powdered nicotinamide (Note 2) and 1 1. of c.p. glacial acetic acid, and the mixture is warmed with occasional shaking on a steam bath until a dear solution is obtained. To this mixture is added 160 ml. (1.39 moles) of cold 30% hydrogen peroxide. An air condenser is attached to the reaction flask, and the mixture is heated on a steam bath for 3.5 hours. 

The reaction mixture is then distilled under reduced pressure (80-100 mm.) (Note 3). After 600-700 ml. has distilled, the mixture is diluted with 150-200 ml. of distilled water and the distillation is continued. The product separates near the end of the distillation, causing somewhat vigorous bumping for a short period. When the bumping has almost ceased, the pressure is reduced to 20 mm. and the distillation continued almost to dryness (Note 4). 

The major portion of wet solid is removed from the flask and transferred to a 1-1. Erlenmeyer flask. The remaining solid is washed out with a little distilled water, and the washings are transferred to the flask. The solid is dissolved in the smallest amount of boiling water required, the flask removed from the heat source, and 50 ml. of ethyl alcohol added (Note 5). The flask is allowed to cool slowly, and, after the major portion of the product has separated, the flask is cooled to 5° overnight. The solid is removed by filtration and washed with cold alcohol, then acetone, and finally ether. The white, crystalline, air-dried product weighs 82-93 g. (73-82%), m.p. 291-293° dec. (rapid heating) the compound starts to turn brown at about 280-285° (Note 6). 

Since hydrogen peroxide attacks rubber stoppers, glass-jointed equipment is recommended. 

For the distillation, the still head consisted of a Claisen-type adapter with a parallel side arm (24/40 standard taper joints), 21 cm. high and 10.5 cm. wide. An ordinary straight still head is attached to the parallel side arm. This large still head prevents any bumping solid from entering the condenser. 

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The procedure given is essentially that described by Taylor and Crovetti.3 Nicotinamide-1-oxide (m.p. 275-276° dec.) has also been prepared by the alkaline hydrolysis of nicotinonitrile-1-oxide 4 and by the action of ammonium hydroxide on methyl nicotinate-l-oxide.6 The melting point of the product prepared by the latter synthesis is reported to be 282-284° dec. 

The modification of cyano groups during the N-oxidation of cyanopyri-dines (p. 198) produces different results according to the position of the cyano group. Thus, with hydrogen peroxide at pH 7 5-8, picolinonitrile gives picolinamide 1-oxide (70 per cent), nicotinonitrile forms nicotinamide 1-oxide (44 per cent) and isonicotinonitrile yields isonicotinamide (45 per cent) with only 4 per cent of the oxide. The first result is ascribed to intramolecular oxidation by the peroxycarboximidic acid, the second to similar intermolecular oxidation. The small proportion of 1-oxide from the 4-isomer may be due merely to deactivation by the 4-substituent . ... 

Oxidation of nicotinamide Oxidation of unsaturated fatty acids Oxidation of lecithin... 

Like pyridine, pyridine homologues and derivatives are converted into amine oxides. a-Picoline oxide is prepared by oxidation with hydrogen peroxide in acetic acid in 83% yield [1188]. Under identical conditions, 2,5-dimethylpyridine oxide and nicotinamide oxide are obtained in 84% yield [160] and 73-82% yield [162], respectively. Pentachloropyridine is oxidized to its oxide by peroxyacetic or peroxytrifluoroacetic acid (equation 504) [755]. 

Ethyl nicotinate upon treatment with concentrated ammonia solution yields nicotinamide, which gives p-cyanopjTidine upon heating with phosphoric oxide ... 

P-Cyanopyridine. Mix 25 g. of powdered nicotinamide with 30g. of phosphoric oxide in a 150 ml. distilling flask by shaking. Immerse the flask in an oil bath and arrange for distillation under a pressure of about 30 mm. Raise the temperature of the oil bath rapidly to 300°, then remove the oil bath and continue the heating with a free flame as long as a distillate is obtained. The nitrile crystallises on cooling to a snow-white solid. Redistil the solid at atmospheric pressure practically all of it passes over at 201° and crystallises completely on cooling. The yield of p-cyanopyridine, m.p. 49°, is 20 g. 

FIGURE 15 5 Structure of NAD the oxidized form of the coenzyme nicotinamide adenine dinucleotide The functional part of the coen zyme is framed in red... 

Insects poisoned with rotenone exhibit a steady decline ia oxygen consumption and the iasecticide has been shown to have a specific action ia interfering with the electron transport iavolved ia the oxidation of reduced nicotinamide adenine dinucleotide (NADH) to nicotinamide adenine dinucleotide (NAD) by cytochrome b. Poisoning, therefore, inhibits the mitochondrial oxidation of Krebs-cycle iatermediates which is catalysed by NAD. 

Glucose [50-99-7] urea [57-13-6] (qv), and cholesterol [57-88-5] (see Steroids) are the substrates most frequentiy measured, although there are many more substrates or metaboUtes that are determined in clinical laboratories using enzymes. Co-enzymes such as adenosine triphosphate [56-65-5] (ATP) and nicotinamide adenine dinucleotide [53-84-9] in its oxidized (NAD" ) or reduced (NADH) [58-68-4] form can be considered substrates. Enzymatic analysis is covered in detail elsewhere (9). 

Indicators There are certain compounds that are suitable as indicators for sensitive and specific clinical analysis. Nicotinamide adenine dinucleotide (NAD) occurs in oxidized (NAD" ) and reduced (NADH) forms. Nicotinamide adenine dinucleotide phosphate (NADP) also has two states, NADP" and NADPH. NADH has a very high uv—vis absorption at 339 nm, extinction coefficient = 6300 (M cm) , but NAD" does not. Similarly, NADPH absorbs light very strongly whereas NADP" does not. 

Pyridine carboxamide [98-92-0] (nicotinamide) (1) and 3-pyridine carboxylic acid [59-67-6] (nicotinic acid) (2) have a rich history and their early significance stems not from their importance as a vitamin but rather as products derived from the oxidation of nicotine. In 1867, Huber prepared nicotinic acid from the potassium dichromate oxidation of nicotine. Many years later, Engler prepared nicotinamide. Workers at the turn of the twentieth century isolated nicotinic acid from several natural sources. In 1894, Su2uki isolated nicotinic acid from rice bran, and in 1912 Funk isolated the same substance from yeast (1). 

In oiological systems, the most frequent mechanism of oxidation is the remov of hydrogen, and conversely, the addition of hydrogen is the common method of reduc tion. Nicotinamide-adenine dinucleotide (NAD) and nicotinamide-adenine dinucleotide phosphate (NADP) are two coenzymes that assist in oxidation and reduction. These cofactors can shuttle between biochemical reac tions so that one drives another, or their oxidation can be coupled to the formation of ATP. However, stepwise release or consumption of energy requires driving forces and losses at each step such that overall efficiency suffers. 

Oxidation of P-nicotinamide adenine dinucleotide (NADH) to NAD+ has attracted much interest from the viewpoint of its role in biosensors reactions. It has been reported that several quinone derivatives and polymerized redox dyes, such as phenoxazine and phenothiazine derivatives, possess catalytic activities for the oxidation of NADH and have been used for dehydrogenase biosensors development [1, 2]. Flavins (contain in chemical structure isoalloxazine ring) are the prosthetic groups responsible for NAD+/NADH conversion in the active sites of some dehydrogenase enzymes. Upon the electropolymerization of flavin derivatives, the effective catalysts of NAD+/NADH regeneration, which mimic the NADH-dehydrogenase activity, would be synthesized [3]. 

Section 15.11 Oxidation of alcohols to aldehydes and ketones is a common biological reaction. Most require a coenzyme such as the oxidized form of nicotinamide adenine dinucleotide (NAD" ). 

Nicotinamide is an essential part of two important coenzymes nicotinamide adenine dinucleotide (NAD ) and nicotinamide adenine dinucleotide phosphate (NADP ) (Figure 18.19). The reduced forms of these coenzymes are NADH and NADPH. The nieotinamide eoenzymes (also known as pyridine nucleotides) are electron carriers. They play vital roles in a variety of enzyme-catalyzed oxidation-reduction reactions. (NAD is an electron acceptor in oxidative (catabolic) pathways and NADPH is an electron donor in reductive (biosynthetic) pathways.) These reactions involve direct transfer of hydride anion either to NAD(P) or from NAD(P)H. The enzymes that facilitate such... 

Newman, Melvin S., 93 Newman projection, 93 molecular model of, 93 Nicotinamide adenine dinucleotide, biological oxidations with, 625-626 reactions of, 725 structure of, 725, 1044 Nicotinamide adenine dinucleotide (reduced), biological reductions with, 610-611... 

It was shown that dibenzothiophene oxide 17 is inert to 1-benzyl-l,4-dihydro nicotinamide (BNAH) but that, in the presence of catalytic amounts of metalloporphyrin, 17 is reduced quantitatively by BNAH. From experimental results with different catalysts [meso-tetraphenylporphinato iron(III) chloride (TPPFeCl) being the best] and a series of substituted sulfoxides, Oae and coworkers80 suggest an initial SET from BNAH to Fe1 followed by a second SET from the catalyst to the sulfoxide. The results are also consistent with an initial coordination of the substrate to Fem, thus weakening the sulfur-oxygen bond in a way reminiscent of the reduction of sulfoxides with sodium borohydride in the presence of catalytic amounts of cobalt chloride81. 

Polypyrrole shows catalytic activity for the oxidation of ascorbic acid,221,222 catechols,221 and the quinone-hydroquinone couple 223 Polyaniline is active for the quinone-hydroquinone and Fe3+/Fe2+ couples,224,225 oxidation of hydrazine226 and formic acid,227 and reduction of nitric acid228 Poly(p-phenylene) is active for the oxidation of reduced nicotinamide adenine dinucleotide (NADH), catechol, ascorbic acid, acetaminophen, and p-aminophenol.229 Poly(3-methylthiophene) catalyzes the electrochemistry of a large number of neurotransmitters.230... 

Ethanol is oxidized by alcohol dehydrogenase (in the presence of nicotinamide adenine dinucleotide [NAD]) or the microsomal ethanol oxidizing system (MEOS) (in the presence of reduced nicotinamide adenine dinucleotide phosphate [NADPH]). Acetaldehyde, the first product in ethanol oxidation, is metabolized to acetic acid by aldehyde dehydrogenase in the presence of NAD. Acetic acid is broken down through the citric acid cycle to carbon dioxide (CO2) and water (H2O). Impairment of the metabolism of acetaldehyde to acetic acid is the major mechanism of action of disulfiram for the treatment of alcoholism. 

Reduced nicotinamide-adenine dinucleotide (NADH) plays a vital role in the reduction of oxygen in the respiratory chain [139]. The biological activity of NADH and oxidized nicotinamideadenine dinucleotide (NAD ) is based on the ability of the nicotinamide group to undergo reversible oxidation-reduction reactions, where a hydride equivalent transfers between a pyridine nucleus in the coenzymes and a substrate (Scheme 29a). The prototype of the reaction is formulated by a simple process where a hydride equivalent transfers from an allylic position to an unsaturated bond (Scheme 29b). No bonds form between the n bonds where electrons delocalize or where the frontier orbitals localize. The simplified formula can be compared with the ene reaction of propene (Scheme 29c), where a bond forms between the n bonds. 

Figure 11-4. Mechanism of oxidation and reduction of nicotinamide coenzymes. There is stereospecificity about position 4 of nicotinamide when it is reduced by a substrate AHj. One of the hydrogen atoms is removed from the substrate as a hydrogen nucleus with two electrons (hydride ion, H ) and is transferred to the 4 position, where it may be attached in either the A or the B position according to the specificity determined by the particular dehydrogenase catalyzing the reaction. The remaining hydrogen of the hydrogen pair removed from the substrate remains free as a hydrogen ion.

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