Neutral buffer formalin

Compound (Miles Laboratories, Elkhart, IN), snap-frozen, and cut into sections for comparison with paraffin-embedded cell sections (3) FFPE Cell Blocks Six cell pellets were fixed in 10% neutral buffered formalin immediately after harvest, at room temperature for 6,12,24h, 3,7, and 30 days, respectively. For further comparison with the cell model system, recently collected sample of human breast cancer tissues were processed by OCT-embedding and snap-freezing the corresponding routine FFPE block that was obtained from the Norris Cancer Hospital and Research Institute at the University of Southern California Keck School of Medicine (USC). This tissue block was processed routinely (formalin-fixed 24h and processed by automatic equipment). 

Figure 12.1 Typical good quality specimen. Small intestine fixed for 24 h in neutral buffered formalin after grossing at 2 mm. Most nuclei show good chromatin patterns, but cell membranes are indistinct. 

The successful of recovery of RNase A functional activity by a heat-induced AR method suggested the possibility of recovering RNase A immunoreactivity as well. The immunoreactivity of native RNase A and RNase A that was incubated at a concentration of 4 mg/mL in 10% neutral buffered formalin for 1 day and then freed of formaldehyde by dialysis against PBS was compared using capture enzyme-linked immunosorbent assay (ELISA). Selected fractions that... 

Figure 15.10 SDS-PAGE of native RNase A (lane 1) and RNase A incubated in 10% neutral buffered formalin for 9 days (lane 2) or in 5% neutral buffered formalin for 1 day (lane 4). The formalin-treated samples were then demodified for4h inTAE buffer (pH 4) at 65°C 10% formalin oligomers (lane 3) and 5% formalin oligomers (lane 5). M, molecular mass markers in kDa. See Rait et al.11 for details.

Fixation Fix tissue in 10% neutral buffered formalin (or equivalent) by placing slides in a shde rack and incubating them in a staining dish containing the fixative. Upon removal from formahn solution, rinse with tap and double distilled water (ddH20) to remove salts. 

Decant the PBS and carefully add 4 mL of 10% neutral buffered formalin down the side of the tube, overlaying the peUet without causing turbulence (see Note 10). Incubate overnight at 4°C. 

Neutral buffered formalin add 100 mL of 37 0% formalin to 900 mL deionized water, then add 4 g monobasic sodium phosphate and 6.5 g dibasic sodium phosphate. Bonin s fluid Combine 1500 mL picric acid (saturated in deionized water at 21 g/L), 500 mL formalin, and 100 mL glacial acetic acid. 

PF Tissue preservation and fixation F10% neutral buffered formalin OW Organ weights D Davidson s fluid X Organ weight collected M Methanol... 

Fix the embryos with 10% neutral-buffered formalin. Embryos can be stored at 4°G up to at least 6 months. 

Fix the specimen m neutral buffered formalin and embed in wax Cut 3—5 pm sections. 

Surgical breast biopsy specimens are first fixed with neutral buffered formalin (4%) for 4-6 hr, followed by zinc-formalin for 2 hr. Paraffin sections (5 xm thick) are placed on silane-coated slides, dried on a slide warmer (60°C) for 1 hr and then in an oven (60°C) for an additional 1 hr. Deparaffinized sections are digested with 0.1% trypsin in PBS at 37°C for 15 min. The sections are placed in 10 mM citrate buffer (pH 6.0) and transferred into a water bath (80° or 90°C) for 2 hr. After a 20-min cooling period, the sections are rinsed... 

For the HercepTest to be valid, it must be performed exactly according to the manufacturer s directions. The tissue must be fixed in 10% neutral buffered formalin or Bouin s solution. Sections of paraffin-embedded tissues should be cut 3-A xm thick and processed in a standard tissue processor. Epitope retrieval should be carried out in a water bath instead of in a microwave oven. Water is used because it provides more uniform and... 

Figure 7.3 A. Human psoriatic tissue (200x). B. Normal human skin (200x). Both samples were immunostained with caspase 14 antibody. Punch biopsies (4 mm) of normal and psoriatic skin samples were obtained from different patients who provided written, informed consent under an IRB-approved protocol. Samples were fixed in 10% neutral-buffered formalin, paraffin embedded, cut to 5-pm sections, and stained with caspase 14 antibody by immunohistochemistry.

Remove lungs and fix them with neutral buffered formalin... 

The same antigen can be used to demonstrate the importance of fixation and antibody-antigen reactions. Fixation in neutral buffered formalin will result in the destruction of an epitope against which some monoclonal antibodies react. Use of those antibodies would indicate... 

For smaller laboratories, the work involved in validation is often difficult, but there are two alternatives. Users can choose a system with an existing standardized and validated protocol and validated interpretation system. Commercially available kits generally provide these, and when utilized exactly as described in the kit insert, are guaranteed to provide diagnostically useful results. A second option would be to use one of the more common standard systems of fixatives with known antibodies, in which publication data has provided some evidence of functionality. As an example, a laboratory could use a 10% neutral buffered formalin fixation with a standard protocol, followed by a biotin-streptavidin HRP system, using a monoclonal antibody combination called AE1/AE3. This has been proven to be a reliable measure of cytokeratin in tissue sections. 

Table 1. Ten percent neutral buffered formalin, pH 7 (10 percent NBF).

---