Necrosis Microscopic

In the work of Samuelsson and Jonsson,86 thirty lungs from 26 healthy young pigs, weighing 20 to 30 kg, were subjected to ECT. In most experiments, 8-10 V and 50 to 70 mA were used and platinum electrodes were employed. Both anodic and cathodic sites showed coagulation necrosis and thrombosis in the vessels when examined microscopically. The lesions healed with fibrotic scarring. 

At necropsy, livers were removed, weighed, examined macroscopically, and prepared for microscopic evaluation. Exposure to 90 ppm chloroform resulted in increased relative liver weights. Female mice exposed to chloroform for 4 days experienced a dose-dependent mild response of uniform hepatocyte lipid vacuolization. Scattered individual hepatocyte necrosis also occurred in a dose-dependent manner. 

Additional studies of decalin exposure in rats have characterized the specific sequence of renal alterations first the variable occurrence of light-microscopically evident proximal convoluted tubule epithelial cell necrosis, presumably a reflection of cellular injury associated with excessive protein accumulation (hyaline droplets) then the occurrence of granular casts at the junction of the inner and outer bands of the outer zone of the medulla and finally, chronic nephrosis, occurring secondary to tubular obstruction by granular casts. It is not... 

Like many volatile halocarbons and other hydrocarbons, inhalation exposure to carbon tetrachloride leads to rapid depression of the central nervous system. Because of its narcotic properties, carbon tetrachloride was used briefly as an anesthetic in humans, but its use was discontinued because it was less efficacious and more toxic than other anesthetics available (Hardin 1954 Stevens and Forster 1953). Depending on exposure levels, common signs of central nervous system effects include headache, giddiness, weakness, lethargy, and stupor (Cohen 1957 Stevens and Forster 1953 Stewart and Witts 1944). Effects on vision (restricted peripheral vision, amblyopia) have been observed in some cases (e.g., Johnstone 1948 Smyth et al. 1936 Wrtschafter 1933), but not in others (e.g., Stewart and Wtts 1944). In several fatal cases, microscopic examination of brain tissue taken at autopsy revealed focal areas of fatty degeneration and necrosis, usually associated with congestion of cerebral blood vessels (Ashe and Sailer 1942 Cohen 1957 Stevens and Forster 1953). 

Traditional endoscopic and surgical procedures provide whole tumor samples well suited for microscopic examination and analysis in the pathology laboratory. The use of whole tissue tumor biopsies for proteomic studies has, however, raised several important issues that have been well demonstrated in CRC [9]. These include cellular heterogeneity in the different bowel parietal layers (mucosa, submucosa, muscularis mucosa, serosa) that may or may not be infiltrated, epithelial cell diversity in the mucosa itself, tissue infiltration by inflammatory cells such as lymphocytes, contamination with other body fluids, and protein degradation following tumor necrosis. In fact, epithelial cell content was found to vary between 9 and 67% in whole biopsies of normal mucosa and between 7 and 95% in tumor biopsies [10]. This study clearly demonstrates the likelihood of large cellular variation between tissue samples. 

Examine sections microscopically for evidence of glomerular hypercellularity and necrosis, mesangial thickening, formation of glomerular crescents, interstitial infiltrates, and development of glomerular and interstitial fibrosis, comparing NTS-treated mice to those treated with normal size and shape (NSS) mice (Fig. 2). 

Figure 16.7. Microscopic appearance of necrosis. (A) Coagulative necrosis in a virally infected avian liver. Hepatocytes in the lower half of the photo are in various stages of necrosis, with small, pyknotic or fragmented nuclei and increased cytoplasmic eosinophilia. (B) Necrotic cells in immune-mediated skin disease, canine. The central cell has a pyknotic nucleus and intensely eosinophilic cytoplasm, while the cells at lower left and upper left are injured and swollen. The smaller cells are neutrophils. See color insert.

Table 33 showed that of the ten animals in the untreated group, eight developed minute tubercles in the liver, five in the spleen and lungs, and nine in the peritoneum and intestines microscopic necrosis suggestive of caseation was found in the liver of three animals and in the spleen, peritoneum, and intestine each in one animal. Smears of acid-fast bac-... 

Ferrans, V.J., Hibbs, R.G., Walsh, J.J., Burch, G.E. (1969). Histochemical and electron microscopic studies on the cardiac necrosis produced by sympathomimetic agents. Ann. NY Acad. Sci 156 309-32. 

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