Mycobacterium tuberculosis materials

In the late 1940s Stacey, with the able and enthusiastic assistance of Paul Kent, examined polysaccharide material from Mycobacterium tuberculosis human strain. From heat-killed cells, two stable, serologically specific polysaccharide fractions and a degraded bacterial glycogen were isolated and examined. 

Purified protein derivative (PPD) Material used in the tuberculin skin test, the most common test for exposure to Mycobacterium tuberculosis. 

In contrast to contact hypersensitivity, tuberculin-type hypersensitivity reactions are primarily dermal and result from intradermal injections into the skin. In people that have had tuberculosis or have been exposed to the bacterium through infection or BCG immunization, a cell-mediated immune response to the bacterium develops. When small amounts of tuberculin (a complex mixture of antigenic material derived from Mycobacterium tuberculosis) are subsequently injected into the skin, a localized T cell-dependent inflammatory response develops in the dermis. Within 24-72 h of injection, individuals with prior exposure to the bacterium display a raised, red, indurated area on the skin at the injection site. The lack of a response suggests no prior exposure to the bacterium. 

Fig. 14.97a,b. Tuberculous bursitis. Transverse 10-5 MHz US images obtained at different levels in a patient presenting with a popliteal mass show a Baker cyst filled with solid hyperechoic material (black asterisk) and anechoic fluid (white asterisks). Surgical exploration revealed a caseum-like content of the cyst. Culture was positive for Mycobacterium tuberculosis... 

Materials. The silver composition of the present invention comprised 10 ppm silver in water. The silver composition was evaluated employing a liquid to liquid matrix against Mycobacterium bovis BCG (TMC 1028). This organism causes tuberculosis in animals and can cause tuberculosis in humans. It is used as a stand-in for M. tuberculosis, the major cause of human tuberculosis, as tests have shown it to have a similar susceptibility to M. tuberculosis. The test organism was exposed to the silver composition in duplicate at four exposure times and quantified using membrane filtration. 

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