Simultaneous multiple peptide synthesis

Houghten, R. A., DeGraw, S. T., Bray, M. K., Hoffmann, S. R., and Frizzell, N. D. (1986) Simultaneous multiple peptide synthesis The rapid preparation of large numbers of discrete peptides for biological, immunological, and methodological studies. BioTechniques 4, 522-528. 

Frank, R. and Coring, R. (1988) Simultaneous multiple peptide synthesis under continuous flow conditions on cellulose paper discs as segmental solid supports. 

Schnorrenberg, G. and Gerhardt, H. (1989) Fully automatic simultaneous multiple peptide synthesis in micromolar scale Rapid synthesis of series of peptides for screening in biological assays. Tetrahedron 45, 7759-7764. 

RA Houghten, ST DeGraw, MK Bray, SR Hoffmann, ND Frizzell. Simultaneous multiple peptide synthesis. Biotechniques 4 522-528, 1986. 

R Frank. Strategies and techniques in simultaneous multiple peptide synthesis based on the segmentation of membrane type supports. Bioorg Med Chem Lett 3 425-430, 1993. 

In addition to the parallel preparation of individual peptide sequences, simultaneous multiple peptide synthesis is used together with the divide, couple, recombine method (9) (also termed spiit-and-mix (10) or porlioning-and-mixing (11)) to prepare peptide combinatorial libraries containing mixtures of thousands to millions of peptides (12). 

Figure2. The principal steps in simultaneous multiple peptide synthesis using the T-bag technology. The resin-containing bags are treated together for washing and deprotection steps, whereas individual couplings are performed in parallel as required for the different peptide sequences.

The Multipin method is an effective, low cost, simultaneous multiple peptide synthesis technology which gives researchers ready access to large... 

Bray, A. M., Maeji, N. J., Jhingran, A. G., and Valerio, R. M. (1991) Gas Phase Cleavage of Peptides from a Solid Support with Ammonia Vapour—Application in Simultaneous Multiple Peptide Synthesis. Tetrahedron Lett. 32, 6163-6166. 

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