Mouse brain cells from

Figure 3. Time course of incorporation of ssSOi>" into cerebroside sulfate (cer-SOk) and sulfogalactosyl glycerol lipid (SGG-lipid) in dissociated brain cells from 15-day mouse embryos grown 19 days in culture...

Tamplin et. al. (54) observed that V. cholerae and A. hydrophila cell extracts contained substances with TTX-like biological activity in tissue culture assay, counteracting the lethal effect of veratridine on ouabain-treated mouse neuroblastoma cells. Concentrations of TTX-like activity ranged from 5 to 100 ng/L of culture when compared to standard TTX. The same bacterial extracts also displaced radiolabelled STX from rat brain membrane sodium channel receptors and inhibited the compound action potential of frog sciatic nerve. However, the same extracts did not show TTX-like blocking events of sodium current when applied to rat sarcolemmal sodium channels in planar lipid bilayers. 

CTx that has been purified from muscles of Gymnothorax javanicus stimulates the release of neurotransmitters such as 7-aminobutyric acid and dopamine from rat brain nerve terminals. It causes a membrane depolarization of mouse neuroblastoma cells and, under appropriate conditions, it creates spontaneous oscillations of... 

Examples using KC1 that require desalting include the identification of the human saliva proteome (Wilmarth et al., 2004), the B. infantis proteome (Vitali et al., 2005), phosphoproteins from HeLa cells (Beausoleil et al., 2004), and phosphoproteins from mouse brain (Ballif et al., 2004). One of the important points to consider is that... 

Proulx [30] summarized the published lipid compositions of BBM isolated from epithelial cells from pig, rabbit, mouse and rat small intestines. Table 3.1 shows the lipid make-up for the rat, averaged from five reported studies [30], On a molar basis, cholesterol accounts for about 50% of the total lipid content (37% on a weight basis). Thus, the cholesterol content in BBM is higher than that found in kidney epithelial (MDCK) and brain endothelial cells (Table 3.1). Slightly different BBM lipid distribution was reported by Alcorn et al. [31] here, the outer (luminal) leaflet of the BBM was seen to be rich in sphingomyelin content, while the inner leaflet (cytosol) was rich in PE and PC. Apical (brush border) and basolateral lipids are different in epithelia. The basolateral membrane content (not reported by... 

Yandava, B. D., Billinghurst, L. L. and Snyder, E. Y. Global cell replacement is feasible via neural stem cell transplantation evidence from the dysmyelinated shiverer mouse brain. Proc. Natl Acad. Sci. U.S.A. 96 7029-7034,1999. 

The toxicity of C60 has been found to be related to its ability to cause oxidative stress (Oberdorster, 2004 and Sayes et al., 2005, 2007). However, literature describing the toxicity of C60 is contradictoiy. The first report on C60 cytotoxicity originated from Tsuchiya et al. who found that C60 inhibited cell proliferation and differentiation dose-dependently in mouse midbrain cells treated at -400 pg/ml for six days. Tsuchiya et al. proposed that reactive oxygen species (ROS) contributed to C60 cytotoxicity. The ROS generation and embryo head abnormalities suggested that C60 may contribute to brain and neuronal diseases such as Down syndrome, Alzheimer s, and Parkinson s disease (Tsuchaiya, 1996). The research that... 

A cell line is a continuously growing cell population, which is derived from tumor cells or from primary cells, which have been immortalized using appropriate vectors. There are several lines of blood-brain barrier endothelial cells available, for example, MBECs (mouse brain endothelial cells), RBE4 cells (rat brain endothelial cells, clone 4), PBMECs (porcine brain microvessel... 

Rietze RL, Valcanis H, Brooker GF, Thomas T, Voss AK, et al. 2001. Purification ofa pluripotent neural stem cell from the adult mouse brain. Nature 412 736-739. 

Excitement about ES-derived cells has been fueled by several reports of laboratory and animal studies. In Parkinson s disease, symptoms result from a loss of cells that produce a critical signal molecule. Cells that produce the signal were created in the lab from mouse ES cells. When injected into the brain, they reduced symptoms in mice with a form of Parkinson s disease. Strains of rats and mice have been developed or discovered that are unable to produce myelin, the fatty insulation that normally covers the nerve fibers of the brain and spinal cord. Injection of insulation-producing cells generated from mouse ES cells produced myelin on the nerve fibers of these animals. 

MAO inhibition. 2-Naphthylamine from smoke, in cell culture, inhibited mouse brain MAO A and B by mixed competitive-and noncompetitive-type inhibition . Mastocytoma induction. Cigarette smoke condensate suspensions ( tars ) from differ-... 

This study reports the first isolation and characterization of neural progenitor and stem cells from the adult brain. Neural progenitor and stem cells were isolated from mouse striatal tissue containing the subventricular zone (a neurogeneic area of the adult brain) and cultured in vitro in the presence of epidermal growth factor. 

EGF-responsive neural stem cells isolated from rat and mouse brain are capable of differentiating into oligodendrocytes and of forming myelin following transplantation into the myelin deficient rat. Duncan, I.D., Archer, D.R., Hammang, J.P. (1993). Abstr Soc Neurosci, 19 (1-3) Abs 689. 

Reisolation of continuously regenerating human neural stem/progenitor AC 133+ cells from transplanted mouse brain. Uchida, N., He, D., Buck, D., Reitsma, M., Masek,... 

Gritti, A., Parati, E.A., Cova, L., Frolichsthal, P., Galli, R., Wanke, E., Faravelli, L., Morassutti, D.J., Roisen, F., Nickel, D.D., Vescovi, A.L. (1996). Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. JNeurosci, 16, 1091-100. 

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