Molecular-weight analyzer, development

The Tulane group developed a MATS instrument specifically to meet the demanding environment of ACOMP, and Brookhaven Instruments Corporation (BIC, Holtsville, NY) took a license from Tulane for this design and currently produces the Bl-MWA (Molecular Weight Analyzer) for ACOMP, size exclusion chromatography (SEC), and batch applications (http //www.bic.com/BI-MwAmw.html). 

ESI has become the most commonly used interface for LC/MS. It was recognized by John Fenn and co-workers as an important interface for LC/MS immediately after they developed it as an ionization technique for MS. ESI transforms ions in solution to ions in the gas phase and may be used to analyze any polar molecule that makes a preformed ion in solution. The technique has facilitated the ionization of heat-labile compounds and high-molecular-weight molecules such as proteins and peptides. ESI is a continuous ionization method that is particularly suitable for use as an interface with FiPLC. It is the most widely accepted soft-ionization technique for the determination of molecular weights of a wide variety of analytes and, has made a significant impact on drug discovery and development since the late 1980s. 

The commercial value of high-performance polymers, i.e., polymers of high molecular weight, which may be composite structures and do not dissolve, have driven the development of new methods for material characterization. Solid-state NMR spectra can be acquired in either high resolution or wide-line mode, methods that yield complementary information [8,13,19,23]. Solid-state NMR has the advantage of being able to analyze the material as received consequently, it... 

Chaif, B. T., and S. B. H. Kent, Weighing naked proteins Practical, high-accuracy mass measurement of peptides and proteins. Science 257 1885-1893, 1992. Proteins with molecular masses of as much as 100 kd or more can be analyzed at picomole sensitivities to give simple mass spectra corresponding to the intact molecule. This development has allowed unprecedented accuracy in the determination of protein molecular weights. 

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