Microliter plate

Precoat the microliter plate with the antigen of interest... 

An exceptional feature of competitive heterogeneous tests, which are at present the only tests that are sufficiently sensitive, is evaluation by photometrical readout of the microliter plates. 

Activity of plasmin-like enzymes was measured with synthetic peptide substrate -Tos-Gly-Pro-Lys-p-nitroanilide ("Serva", Germany). Tear samples (5-25 (jl ) were placed into microliter plate wells. 150 fil of 0.05 M K-phosphate (pH 7.9) and 40 /il of substrate (2.4 mg/ml) were added in plate well for reaction initiation. Microtiter plate was diermostated at 250 °C. Optical density changes were measured using Microrider (Uniscan 11, Labsystems, Finland) at 405 nm. Measurements were made for 3-4 times during 5 hours. Plasmin-like activity of tear was calculated as tangent of slop of line in coordinates optical density via time. For accurate calculation we used a special software -Enzfitter (version 1.05 - EGA). We determined arbitrary unit of plasmin-like activity of tear as change of 1.0 unit of optical density per minutes per 1 ml of tear. 

Amperometric Tea and fruits Ethanolic phosphate buffer (EPBS, 40% ethanol, pH 7.4) pencil-rod working electrode 100 mV 0.2 mM TEAC microliter plate automated system Intarakamhang and Schulte (2012)... 

An alternative to TBP distillation is simulated distillation by gas chromatography. As described by Green, Schmauch, and Worman [Anal. Chem., 36, 1512 (1965)] and Worman and Green [Anal. Chem., 37, 1620 (1965)], the method is equivalent to a 100-theoretical-plate TBP distillation, is veiy rapid, reproducible, and easily automated, requires only a small microliter sample, and can better... 

Contact angle measurements were obtained using a goniometer, measuring the advancing angle from 2 to 20 microliter drop sizes, of purified water upon polymer films at room temperature. Films were cast on metal plates and allowed to dry slowly from chloroform solutions. Several spots were measured on each film and the results averaged. 

Ten microliters of ice-cold master mix is distributed to a 96-well conical plate using a multidrop. Compounds (0.1 /il) are transferred from 2 mM (100% DMSO) daughter plates into translation reactions using stainless steel pins. Between transfers, pins are washed with RNAse-free deionized water, ethanol, and 100% DMSO, and dried on a clean 3 MM Whatman paper. 

We have found that many compounds identified in our screen are nonspecific inhibitors of luciferase enzyme activity. To eliminate these, we test the hits in a luciferase enzyme-based counterscreen. Firefly and renilla luciferase are produced in vitro by programming Krebs-2 extracts with FF/HCV/Ren mRNA and allowing the translations to proceed at 30° for 1 h. Ten microliters are then pipetted into a 96-well plate and compound is added to a final concentration of 20 /iM (1% DMSO). Luciferase activity is then determined as described previously in step 3. Since compound is added only after the translation reaction is complete, inhibitors of translation should not score positively in this assay. Typically, a 1-ml in vitro translation reaction is sufficient to screen 45 candidate hits in duplicate for nonspecific luciferase inhibitory activity. Compounds that inhibit in this counterscreen are eliminated from future analysis. 

In order to control the quantity of fullerene, contacting biological objects, FoS were obtained by evaporation of saturated solution of C60 in hexane introduced in the wells of 96-well culture plates ( Sarstedt ). Twenty-five microliters of solution was applied to each well and evaporated at 20-25 °C, after which the procedure was repeated several times to obtain a desirable concentration of fullerene (10, 20, and 30pg/cm2). Application of such volume allows obtaining a surface, covered with fullerene on the bottom and partly on the walls of a well at a high less than 2 mm. Microscopic investigations (optical and electronic microscopy) have shown that the surface was covered irregularly fullerene formed the isolated clusters, so that obtained fullerene films were not the real films, but rather isolated clusters of fullerene molecules (data not shown). However, it should be noted that their dimensions were smaller than those of cells and each cell covered several such clusters. 

Assuming an exclusion volume of 5 ml per column allows construction of Table I from Equation 1. Table I lists the bandwidth in microliters as a function of column plate number and the number of columns in series. The data assume that the plate number may be generated at total exclusion, as well as at total permeation actual measurements made using the smaller pore size column si ibstantiate this. 

A miniaturized circular chromatographic technique on a TLC plate can be conveniently used to select an appropriate mobile phase [HT]- A few microliters of the sample solution are spotted on the plate. After the evaporation of the solvent in the center of each spot a few miefoliters of a pure eluent or an eluent mixture is transferred by using a glass capillary or a fine-tipped medicine dropper. On a S x 20 cm TLC plate, about 10 different spots can be applied and, of course, the same number of different eluents can be checked. If the elution strength of the test fiiixliires is too... 

The most common and simplest procedure is to place a few microliters of the test solution over a small puncture wound on a detached leaf. The puncture wound enhances the access of the toxin to the leaf tissue. The leaf is then placed in a petri dish containing a filter paper saturated with water. The top cover of the plate is sealed with parafilm, and the plate is incubated under controlled light and temperature conditions. Toxin activity is usually indicated by chlorotic, necrotic, or colored spots on the leaf. Other methods for bioassay involving CO2 fixation, or effects on organelles, whole plants, protoplasts, tissue cultures, or plant parts are outlined (, 7). 

Fifteen microliters of JM109 competent cells and 1.5 pL ofthe ligation reaction mixture are mixed in a 96-well plate followed by sealing with plate sealers, and the mixture is left on ice for 30 min. Then heat-shock is applied to the cells by placing the 96-well plate at 42°C for 45 s. The plate is then immediately placed on ice for 2 min see Note 3), and then each transformation reaction mixture is transferred into 150 pL of SOC medium in a 96-well deep well culture plate. The plate is sealed with an air-permeable sheet and incubated at 37°C for 90 min with gentle shaking. 

One hundred microliters of the culture is spotted onto a square LB agar plate containing 50 pg/mL of kanamycin. Several spots and one streak are formed on the LB agar plate for each... 

Eighty microliters of 70% ethanol is added to each well. The plate is centrifuged at 2,380 g at 4°C for 30 min, and the supernatants are discarded by reversing the plate. Immediately, the residual liquids are completely removed from the plate as described above. The samples are dried by keeping them at room temperature for 10 min and dissolved into 10 pL of HjO. These are the donor DNA solutions. ... 

Thirty microliters of the suspension is spread on one quarter of a square LB agar plate containing 50 pg/mL of kan-amycin and incubated at 37°C overnight. Thus four kinds of samples are on one plate and 24 plates are needed per a 96-sample set. In addition, glycerol stocks are prepared by blending 100 pL of the cell suspension with 50 pL of Cell Stock Buffer in a 96-well format. 

For glycerol stock, 50 pL of the culture is added to 25 pL of Cell Stock Buffer in a 96-well plate. The remaining suspensions are applied to plasmid preparation using the Wizard SV 96 Plasmid DNA Purification System see Note 6). One microliter of resultant plasmid solution is applied to agarose gel electrophoresis to examine the yields of plasmids. 

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