Immobilized Microfluidic Enzyme Reactor

Enzymatic bioreactor Immobilized enzyme biosensor Immobilized enzyme reactor Immobilized microfluidic enzyme reactor (IMER) Membrane reactor... 

A wide variety of applications for microfluidic bioreactors have been developed. One of the most common is immobilized enzyme reactors that are used for applications such as biosensors, medical diagnosis, and synthetic applications. Most of the... 

Microfluidic bioreactors are a variety of devices that can be made by immobilizing a variety of biologically active substrates within a microfluidic device [1]. The ability to create a variety of biologically important devices is critical to enabling the true total analytical system. The variety of devices that can be made in this way ranges from immobilized enzyme reactors to enzymatic biosensors, immunoassys, and affinity chromatographic stationary phases. In order to form a microfluic bioreactor it is necessary to immobilize the active molecule within the device either directly onto the channel or onto a solid support within the channel such as a bead or a monolith. 

To fulfill such requirements, attempts have been made in the past decade by researchers working on peptide mapping and proteomics through development of immobilized microfluidic enzymatic reactors. Microfluidic enzymatic microreactors are an alternative to in-solution method employing immobilization of proteases on microchaimels of chip-based reactors or surfaces of capillaries. The microreactors that enable proteolytic digestion by enzymes immobilized on solid supports are also referred to as immobilized enzyme reactors, IMERs. The great potential of IMERS for proteomic applications comprise rapid and enhance... 

The second device was a multiplate-stacked microfluidic reactor, originally developed by Hessel, Lowe and co-workers for gas-phase transformations [lb] (Figure 3.2) and now adapted for biocatalytic processing with immobilized enzymes. 

Microfluidic bioreactors have a wide range of useful applications that have been demonstrated. The most common application is for immobilized microfluidic enzyme reactors (IMER). IMERs are most commonly used for protein analysis by peptide mapping. Microfluidic bioreactors have also been widely used for biosensing applications. This typically involves immobilizing an enzyme that enables the detection of the analyte of interest. Immunoassays are also a common method to enable the detection of a specific biologically active analyte in a microfluidic system. 

MicroChannel Enzyme Reactor. Enzymatic conversion is receiving attention because it is an environmentally friendly approach to synthesizing chemicals. Reactions can be carried out enzymatically in the solution phase or by immobilizing enzymes on microfluidic walls. The use of the latter type of reaction has the same mass and heat transfer advantages as the use of a microfluidic synthetic reactor. More information can be found in a published review article [6] and in the article microfluidic bioreactors in this encyclopedia. 

Microfluidic Reactors with Immobilized Enzymes for Biocatalytic Transformations... 

Table 10.4 Enzymatic transformations in microfluidic reactors with immobilized enzymes.

A simple microfluidic reactor system He et al. [124] for the effective synthesis of enzyme-functionalized nanoparticles offers many advantages over batch reactirais, including excelloit enzyme efficiencies. Better control of the process parameters in the microfluidic reactor system ovct batch-based methodologies enables the production of silica nanoparticles with the optimum size for efficient enzyme immobilization with long-term stability. The synthetic approach used glucose oxidase and two different nucleation catalysts of similar molecular mass the natural R5 peptide, and PEI polymer... 

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