Methanobacterium thermoautotrophicum

Methanobacterium thermoautotrophicum Methanosarcina barkeri Micromonospora purpurea Nocardia gardneri... 

Tungsten may be incorporated into some proteins of purinolytic Clostridia (Wagner and Andreesen 1987), and into formyhnethanofuran dehydrogenase in Methanobacterium thermoautotrophicum (Bertram et al. 1994). 

Bertram PA, RA Schmitz, D Linder, RK Thauer (1994) Tungsten can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum identification and characterization of a tungsten isoenzyme of formylmethanofuran dehydrogenase. Arch Microbiol 161 220-228. 

Afting, C., Hochheimer, A. and Thauei R. K. (1998) Function of H2-forming methylene-tetrahydromethanopterin dehydrogenase from methanobacterium thermoautotrophicum in coenzyme F420 reduction with H2. Arch. Microbiol., 169, 206-10. 

Alex, L. A., Reeve, J. N., Orme-Johnson, W. H. and Walsh, C. T. (1990) Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H. Biochemistry, 29, 7237-44. 

Coremans, J. M. C. C., Van der Zwaan, J. W. and Albracht, S. P. J. (1989) Redox behaviour of nickel in hydrogenase from Methanobacterium thermoautotrophicum (strain Marburg). Correlation between the nickel valence state and enzyme activity. Biochim. Biopbys. Acta, 997, 256-67. 

Setzke, E., Fledderich, R., lEeiden, S. and Thauer, R. K. (1994) lE2 heterodisulfide oxidoreduc-tase complex from Methanobacterium thermoautotrophicum. Composition and properties. Eur. J. Biochem., 220, 139-48. 

Tersteegen, A. and Hedderich, R. (1999) Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteins. Eur. J. Biochem. 264, 930-43. 

Woo, G., Wasserfallen, A. and Wolfe, R. S. (1993) Methyl viologen hydrogenase II, a new member of the hydrogenase family from Methanobacterium thermoautotrophicum. DELTA. H. J. BacterioL, 175, 5970-7. 

Smith D, Doucette-Stamm RLA, Deloughery C, et al. 1997. Complete genome sequence of Methanobacterium thermoautotrophicum AH fnnctional analysis and comparative genomics. J Bacteriol 179 7135-55. 

Smith KS, Ferry JG. 1999. A plant-type (beta-class) carbonic anhydrase in the thermophilic vaethanoarchaeon Methanobacterium thermoautotrophicum. J Bacteriol 181 6247-53. 

Strop P, Smith KS, Iverson TM, et al. 2001. Crystal structure of the cab -type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. J Biol Chem 276 10299-305. 

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. A structural proteomic project used the sequenced genome of the thermophilic Methanobacterium thermoautotrophicum as a source of targets for structure determination.As expected, proteins from M. thermoautotrophicum possess high thermostability with a transition midpoint temperature between 68 and 98 °C. Small proteins were C- and N-labelled and their solution structures were solved using multinuclear and multidimensional NMR spectro-scopy. The project was also extended to some proteins from Thermotoga maritima ... 

Biological. Methanococcus thermolithotrophicus, Methanococcus deltae, and Methanobacterium thermoautotrophicum metabolized 1,2-dichloroethane releasing methane and ethylene (Belay and Daniels, 1987). 1,2-Dichloroethane showed slow to moderate biodegradative activity with concomitant rate of volatilization in a static-culture flask-screening test (settled domestic wastewater inoculum) conducted at 25 °C. At concentrations of 5 and 10 mg/L, percent losses after 4 wk of incubation were 63 and 53, respectively. At a substrate concentration of 5 mg/L, 27% was lost due to volatilization after 10 d (Tabak et al., 1981). 

Biological. A strain of Acinetobacter sp. isolated from activated sludge degraded ethyl bromide to ethanol and bromide ions (Janssen et al., 1987). When Methanococcus thermolithotrophicus, Methanococcus deltae, and Methanobacterium thermoautotrophicum were grown with H2 CO2 in the presence of ethyl bromide, methane and ethane were produced (Belay and Daniels, 1987). 

The enzymes in the zebra fish pathway are presumably very similar to those of other vertebrates. However a completely different type of GTP cyclohydrolase has been identified in the hyperthermophilic euryarchaeon, Methanococcus jannashii <2002B15074>. This enzyme, in purified recombinant form, produced as a stable end product 2-amino-5-formylamino-6-ribofuranosylamino-4(3//)-pyrimidinone monophosphate, a compound that is an intermediate in the action of normal GTP cyclohydrolases. The biosynthesis of the incorporation of the pterin into methanopterin in Methanobacterium thermoautotrophicum has been proposed to occur via substitution of 7,8-dihydro-6-hydroxymethylpterin diphosphate 227 (Scheme 44) <1998BBA257>. 

Currently, Ni(I) macrocyclic complexes have attracted much attention. This is because Ni(II) tetraaza macrocyclic complexes catalyze the electrochemical reduction of C02 and alkyl halides, and it is proposed that the Ni(I) species are involved in such reactions (1,2, 76-79, 82, 124-126). Furthermore, F430, a Ni(II) hydrocorphinoid complex, is a prosthetic group of methyl coenzyme M reductase that catalyzes the reductive cleavage of S-methyl coenzyme M to methane in the final stage of C02 reduction to methane (127-130). An EPR signal detected in whole cells of Methanobacterium thermoautotrophicum has been attributed to an Ni(I) form of F430 in intact active enzyme (131,132). 

Oberlies, G. Fuchs, G. Thauer, R.K. Acetate thiokinase and the assimilation of acetate in methanobacterium thermoautotrophicum. Arch. Microbiol., 128, 248-252 (1980)... 

Examples of EPR spectra of the Ni hydrogenase from D. gigas are shown in Fig. 4. This hydrogenase, in common with those from Methanobacterium thermoautotrophicum (7, 9, 39) and Chromatium... 

Biosynthesis of 7,8-didemethyl-8-hydroxy-5-deazaflavin starts from guanosine-5 -triphosphate (GTP), which leads through several steps to 5-amino(6-ribitylamino)-2,4( 1 //,3//)-pyrimidinedione 5 -phosphate, whose addition to 4-hydroxybenzoic acid which comes from shikimate gave the target compound <85JA8300>. The biosynthesis of riboflavin and deazaflavins has been studied in Methanobacterium thermoautotrophicum <91JBC9622>. 

Methanobacterium thermoautotrophicum (strain DH). FEMS Microbiol. Lett., 117, 263-268. 

Graf E-G, Thauer RK (1981) Hydrogenase from methanobacterium thermoautotrophicum, a nickel-containing enzyme. FEBS Lett. 136 165-169... 

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