Methanobacterium thermoautotrophicum hydrogenases

Methanobacterium formicicum formate dehydrogenases, 663 Methanobacterium thermoautotrophicum hydrogenases nickel, 647... 

Alex, L. A., Reeve, J. N., Orme-Johnson, W. H. and Walsh, C. T. (1990) Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H. Biochemistry, 29, 7237-44. 

Coremans, J. M. C. C., Van der Zwaan, J. W. and Albracht, S. P. J. (1989) Redox behaviour of nickel in hydrogenase from Methanobacterium thermoautotrophicum (strain Marburg). Correlation between the nickel valence state and enzyme activity. Biochim. Biopbys. Acta, 997, 256-67. 

Tersteegen, A. and Hedderich, R. (1999) Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteins. Eur. J. Biochem. 264, 930-43. 

Woo, G., Wasserfallen, A. and Wolfe, R. S. (1993) Methyl viologen hydrogenase II, a new member of the hydrogenase family from Methanobacterium thermoautotrophicum. DELTA. H. J. BacterioL, 175, 5970-7. 

Examples of EPR spectra of the Ni hydrogenase from D. gigas are shown in Fig. 4. This hydrogenase, in common with those from Methanobacterium thermoautotrophicum (7, 9, 39) and Chromatium... 

Graf E-G, Thauer RK (1981) Hydrogenase from methanobacterium thermoautotrophicum, a nickel-containing enzyme. FEBS Lett. 136 165-169... 

EXAFS studies on the oxidized state of D. gigas hydrogenase are consistent with 2-3 sulfur atoms at 2.21 A and 2-4 at 2.28 A (78) for the Methanobacterium thermoautotrophicum enzyme, the spectrum was interpreted as due to 2.9 sulfur atoms at 2.25 A (79). There is some uncertainty in the number of donors and as to the possible presence of... 

Albracht SPJ, Graf E-G, Thauer RK. 1982. The EPR properties of nickel in hydrogenase from Methanobacterium thermoautotrophicum. FEBS Lett 140 311-313. 

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