Methanosarcina barkeri

Methanobacterium thermoautotrophicum Methanosarcina barkeri Micromonospora purpurea Nocardia gardneri... 

Holliger C, G Schraa, E Stuperich, AJM Stams, AJB Zehnder (1992) Evidence for the involvement of corri-noids and factor F43Q in the reductive dechlorination of 1,2-dichloroethane by Methanosarcina barkeri. J Bacteriol 174 4427-4434. 

The acetyl-coenzyme A decarbonylase synthase complex contains five polypeptide sub-nnits and in acetate-degrading methanotrophs, such as Methanosarcina barkeri and M. thermophila, catalyzes the formation of methane and COj from acetyl-CoA ... 

Under conditions of oxygen limitation in continuous culture, mixed populations of aerobic bacteria and the strictly anaerobic Methanobacterium formicicum and Methanosarcina barkeri could be maintained (Gerritse and Gottschal 1993a). 

Hippe H, D Caspari, K Fiebig, G Gottschalk (1979) Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Proc Natl Acad Sci USA 76 494-498. 

Naumann E, H Hippe, G Gottschalk (1983) Betaine new oxidant in the Stickland reaction and methanogen-esis from betaine and L-alanine by a Clostridium sporogenes-Methanosarcina barkeri coculture. Appl Environ Microbiol 45 474-483. 

Patterson JA, RB Hespell (1979) Trimethylamine and methylamine as growth substrates for rumen bacteria ind Methanosarcina barkeri. Curr Microbiol 3 79-83. 

The methanogen Methanosarcina barkeri is able to transform trichlorofiuoromethane (CFC-11) by successive loss of chlorine to produce chlorofiuoromethane (Figure 7.70a) (Krone and Thaner 1992). A similar transformation has been demonstrated in the presence of sulfate and bntyrate with a mixed cnltnre containing putatively Desulfovibrio baarsii and Desulfobacterpostgatei (Sonier et al. 1994). 

FIGURE 7.70 (a) Metabolism of trichlorofluoromethane by Methanosarcina barkeri, (b) transformation of... 

Krone HE, RK Thauer (1992) Dehalogenation of trichlorofluoromethane (CFC-11) by Methanosarcina barkeri. EEMS Microbiol Lett 90 201-204. 

Mazumder TK, Nishio N, Fukazaki S, et al. 1986. Effect of sulfur-containing compounds on growth of Methanosarcina barkeri in defined medium. Appl Environ Microbiol 10 617-622. 

Fiebig, K. and Friedrich, B. (1989) Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro). Eur. J. Biochem., 184, 79-88. 

Meuer, J., Bartoschek, S., Koch, J., Kunkel A. and Hedderich, R. (1999) Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri. Eur. J. Biochem., 265, 325-35. 

Michel, R., Massanz, C., Kostka, S., Richter, M. and Fiebig, K. (1995) Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro. Eur. J. Biochem., 233, 727-35. 

Vaupel, M. and Thauer, R. K. (1998) Two F420-reducing hydrogenases in Methanosarcina barkeri. Arch. Microbiol., 169, 201-5. 

Vorholt JA, Vaupel M, Thauer RK. 1996. A polyferredoxin with eight [4Fe-4S] clusters as a subunit of molybdenum formyhnethanofuran dehydrogenase from Methanosarcina barkeri. Eur J Biochem 236 309-17. 

Acetyl coenzyme A synthase of C. thermoaceticum has a (aP)2 dimer-of-dimers structure with subunit molecular masses 78 kDa (a) and 71 kDa (P). Analysis indicated 12 Fe, 14 S, 2 Ni, 1 Zn per ap dimer. CO dehydrogenase activity resides in the P subunit (AcsA). This subunit has 46% identity (75% homology) with the R. rubrum C00S protein and appears to contain cluster C. Cluster A is located at least partially in the a subunit (AcsA) ACS of Methanosarcina barkeri comprises five subunits a, 84-93 kDa P, 63 kDa y, 53 kDa 8, 51 kDa , 20 kDa [141], The CODH activity is associated with the a subunit. 

Novak PJ, Daniels L, Parkin GF. Enhanced dechlorination of carbon tetrachloride and chloroform in the presence of elemental iron and Methanosarcina barkeri, Methanosarcina thermophila, or Methanosaeta concilia. Environ Sci Technol 1998 32 1438-1443. 

Kemner, J., and Zeikus, J. G. 1994. Purification and characterization of membrane-bound hydrogenase from Methanosarcina barkeri MS. Arch. Microbiol. 161, 47-54. 

Nishihara, M., and Koga, Y., 1991, Hydroxyarchaetidylserine and hydroxyarchaetidyl-myo-inositol in Methanosarcina barkeri Polar lipids with a new ether core portion. Biochim. Biophys. Acta 1082 211-217. 

Grahame, D. A., and Demoll, E., 1995, Substrate and accessory protein requirements and thermodynamics of acetyl-CoA synthesis and cleavage in Methanosarcina barkeri, Biochem. 34(14) 4617n4624. 

Burke SA, Krzycki JA. Reconstitution of Monomethylamine Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J. Biol. Chem. 1997 272 16570-16577. 

Properties Methanobacterium ihermoautotrophicum Methanosarcina barkeri strain Fusaro [113]... 

Property Methanobacterium thermoautotroph-icum strain AH [100] Methanosarcina barkeri strain MS [186] Methanopyrus kandleri strain MS [356]... 

Methanosarcina barken [43]. While Methanosphaera stadtmanae is restricted to this kind of methanogenesis, Methanosarcina barkeri in addition can disproportionate methanol. Methanolobus tindarius being devoid of a hydrogenase can only disproportionate methanol rather than reduce it with molecular hydrogen [44]. 

As was first shown for Methanosarcina barkeri[A5 methanogens can grow on methylamine, dimethylamine and trimethylamine as sole carbon and energy sources. The various methylamines are disproportionated analogously to methanol in addition ammonia is formed (Reactions 7-9 of Table 1). 

---