Genome-wide scale

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. A structural proteomic project used the sequenced genome of the thermophilic Methanobacterium thermoautotrophicum as a source of targets for structure determination.As expected, proteins from M. thermoautotrophicum possess high thermostability with a transition midpoint temperature between 68 and 98 °C. Small proteins were C- and N-labelled and their solution structures were solved using multinuclear and multidimensional NMR spectro-scopy. The project was also extended to some proteins from Thermotoga maritima ... 

The ability to study certain subjects systematically on a large scale, better to the largest possible extent, rather than on a one-by-one and on a case-by-case basis, is what finally renders a specific technology to mature towards an associated -omics . Just as genomics is the extension of genetics to a genome-wide scale, chemo-... 

Date, S. V. and C. J. Stoeckert. 2006. Computational modeling of the Plasmodium falciparum interactome reveals protein function on a genome-wide scale. Genome Res. 16, 542-549. 

It is interesting to consider how well suited the two-hybrid assay is for its original conception - the discovery of new proteins on the basis of their binding to other known proteins - particularly as this assay begins to be carried out on a genome-wide scale. An important paper that bears on this question,... 

Advances in microarray technology, with the use of cDNA or oligonucleotides immobilized in a predefined organization on a solid phase, have led to measurements of mRNA expression levels on a genome-wide scale (see chapter 3). The results of the measurements can be displayed on a plot (fig. 13.1) on which a row represents one gene at various times, a column the whole set of genes, and the time of gene expression... 

Currently, protein expression mapping is often performed by coupled 2DE or HPLC and MS-MS technologies. However, the development of protein microarrays (protein chips) may provide another powerful method to explore protein expression (protein profiling) and function on a genome-wide scale (Fung, 2004 LaBaer and Ramachandran, 2005 Hultschig et al, 2006). Figure 16.8 shows some potential applications for protein microarrays. 

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