Membrane Reichert

Charych D, Cheng Q, Reichert A, Kuziemko G, Stroh M, Nagy JO, Spevak W, Stevens RC (1996) A litmus test for molecular recognition using artificial membranes. Chem Biol 3 113-120... 

Another complication in the quantitation of TIRF on cells is the effect of the membrane thickness itself on the profile of the evanescent wave. Reichert and Truskey<105) have calculated that, in theory, the thickness of the membrane should have a negligible effect on the fluorescence and that a simplified theory of three stratified layers (glass/water/cytoplasm) should be adequate. The theory approximates for simplicity that scattering plays a negligible role and that fluorescence intensity versus angle of observation and fluorescence lifetime are not functions of distance to the interface z. Experiments that... 

Charych, D., Cheng, Q., Reichert, A., Kuziemko, G., Stroll, N., Nagy, J., Spevak, W., Stevens, R. (1996). A litmus test for molecular recognition using artificial membranes. Chemistry and Biology, 3, 113-120. 

D. Reichert, A. Kuhnle, H.-P. Krimmer and K. Drauz, Julia-Colonna asymmetric epoxidation in a continuously operated chemzyme membrane reactor, Synlett 2002, (5), 707-710. 

Wisniewski N, Moussy F, Reichert WM. Characterization of implantable biosensor membrane biofouling. Fresenius Journal of Analytical Chemistry 2000, 366, 611-621. 

Wisniewski N, Reichert M. Methods for reducing biosensor membrane biofouling. Colloids and Surfaces B. Biointerfaces 2000, 18, 197-219. 

Wisniewski N, Klitzman B, Miller B, Reichert WM. Decreased analyte transport through implanted membranes differentiation of biofouling from tissue effects. Journal of Biomedical Materials Research 2001, 57, 513-521. 

The network structure has been observed by electron microscopy in Reichert s membrane following treatment of tissue sections with plasmin (Inoue et al., 1983). This enzyme removes a major portion of the 3- to 8-nm-thick cords that constitute the major element in the basement membrane, leaving behind a network of fine filaments 1.5 to 2 nm in diameter. Presumably, the cords have a framework consisting of one or more collagen IV filaments that are coated with laminin and with other basement membrane components. 

Chondroitin sulfate was identified in embryonic basement membrane on the basis of studies showing the susceptibility of sulfate-labeled material to chondroitinase and a reaction with monoclonal antibodies (Couchman et al., 1984 Paulsson et al., 1985b). Similarly, Reichert s membrane, endothelial L2, PYS cells, EHS tumor cells, and isolated glomeruli produce variable amounts of these proteoglycan (Oohira et al., 1983 Stow et al., 1983 Oldberg et al., 1981 Tyree et al., 1984 Hassell et al., 1980). The molecule produced by L2 cells (Mr 40K) contains a small protein core with a unique amino acid sequence (Bourdon et al., 1985). It appears that in such systems, little of the chondroitin sulfate proteoglycan is deposited in the matrix. 

Grasso P, Heindel JJ, Powell CJ, Reichert LE Jr (1993) Effects of mono(2-ethylhexyl)phthalate, a testicular toxicant, on follicle-stimulating hormone binding to membranes of cultured rat Sertoli cells. Biol Reprod 48 454-459 Ketelslegers JM, Catt KJ (1974) Receptor binding properties of 125-hFSH prepared by enzymatic iodination. J Clin Endocrin Metab 39 1159-1162... 

Errede LA, Jefson GB, Langager BA, Olson PE, Ree BR, Reichert ME, Sinclair RA, Stofko JJ (1988) Reactive microporous composite membranes. In Leyden DE, Collins WT (eds), Chemically modified surfaces in science and industry, vol 2 Proceedings of the chemically modified surfaces symposium, Fort Collins, Colorado, June 17-19 1987. Gordon and Breach Science Publishers, New York, p 91... 

Kim, J.W., Wieckowski, E., Taylor, D.D., Reichert, T.E., Watkins, S. and Whiteside, TL. (2005) Fas ligand-positive membranous vesicles isolated from sera of patients with oral cancer induce apoptosis of activated T lymphocytes. Clin. Cancer Res. 11, 1010-1020. 

Reichert, J., Grasnick, D., Afonin, S., et al. (2007) A critical evaluation of the conformational requirements of fusogenic peptides in membranes. European Biophysics Journal with Biophysics Letters, 36, 405 413. 

Branten AJ, Reichert LJ, Koene RA, et al. Oral cyclophosphamide versus chlorambucil in the treatment of patients with membranous nephropathy and renal insufficiency. QJM 1998 91 359-366. 

Bornhovd, C., Vogel, F., Neupert, W. and Reichert, A.S., Mitochondrial membrane potential is dependent on the oligomeric state of FI FO-ATP synthase supracomplexes, J Biol Chem 281(2006)13990-13998. 

The small sphere enclosed within the trophoblast proves from its staining reaction to be the Reichert membrane, which normally intervenes between the fetal membrane and the trophoblast. It is now found to enclose merely a few disorganized embryonic cells. 

Reichert, A., J.O. Nagy, W. Spevak, and D. Charych. 1995. Polydiacetylene liposomes functionalized with sialic-acid bind and colorimetrically detect influenza-vims. J Am Chem Soc 117 (2) 829-830. Charych, D., Q. Cheng, A. Reichert, G. Kuziemko, M. Stroh, J.O. Nagy, W. Spevak, and R.C. Stevens. 1996. A Htmus test for molecular recognition using artificial membranes. Chem Biol 3 (2) 113-120. 

Fig. 3. The decidua and thin Reichert s membrane overlying the El 1 yolk sac are trimmed back level with the placenta.

Next, open Reichert s membrane although this is thin and transparent, it is mostly overlain with a layer of trophoblast and blood cells, and it may be necessary to pick through this layer before rupturing Reichert s membrane, after which removal is straightforward, trimming it up to, but not beyond, the placental border (Fig. 8). 

Fig. 8. The Reichert s membrane of the E9 embryo is opened using watchmaker s forceps, and the surplus is trimmed to the border of the placenta.

Fig. 12. Reichert s membrane is opened with finely ground watchmaker s forceps.

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