Influenza colorimetric detection

Fig. 8 (top) Liposome with polydiacetylene linked monolayer mixed with ligand for receptor detection. (bottom) Colorimetric detection of influenza virus using polymerized liposomes to which have been added increasing amounts of influenza virus from left to right. [18]... 

Reichert A, Nagy JO, Spevak W, Charych D (1995) Polydiacetylene liposomes functionalized with sialic-acid bind and colorimetrically detect influenza-virus. J Am Chem Soc 117 829-830... 

Figure 12.3 Colorimetric detection of influenza by sialoside bilayer assembly (2% sialoside lipid 2 and 98% matrix lipid 1). (a) The colorimetric response of the film, supported on a glass microscope slide, is readily visible to the naked eye for quahtative evaluation of the presence of virus. The film on the left (blue) has been exposed to a blank solution of PBS. The film on the right (red) has been exposed to 100 hemagglutinin units (HAU) of vims (CR = 77%). (b) The...

Figure 12.4 Colorimetric detection of influenza virus using polymerized liposomes containing sialic acid, (a) Photograph of liposomes to which have been added increasing amounts (from left to right) of influenza virus. Liposomes were 1 and 95% 5. To each well was added the following amounts of influenza virus (left to right) 0, 8, 16, and 32 hemagglutinin units (HAU). Reprinted from Charych et al. (1996). Copyright 1996 Elsevier Science. (See color insert.)...

Back MG, Stevens RC, Charych DH. Design and synthesis of novel glycopol3thiophene assembhes for colorimetric detection of influenza vims and E. coli. Bioconjug Chem 2000 11 777-788. 

Reichert, A., J.O. Nagy, W. Spevak, and D. Charych. 1995. Polydiacetylene liposomes functionalized with sialic-acid bind and colorimetrically detect influenza-vims. J Am Chem Soc 117 (2) 829-830. Charych, D., Q. Cheng, A. Reichert, G. Kuziemko, M. Stroh, J.O. Nagy, W. Spevak, and R.C. Stevens. 1996. A Htmus test for molecular recognition using artificial membranes. Chem Biol 3 (2) 113-120. 

Baek, M. G., Stevens, R. C. Charych, D. H. (2000). Design and synthesis of novel glycopolythiophene assemblies for colorimetric detection of influenza virus and E-coli. Bioconjugate Chem., 11, 777-788. 

Charych et al. reported a direct colorimetric assay for the detection of influenza virus, using a polydiacetylene bilayer immobilized on a glass microscope slide and functionalized with sialic acid (the vims receptor molecule). Upon binding of the vims, a color change is visible to the naked eye, but could also be quantified by absorption spectroscopy. The authors demonstrated by physical characterization that the packing of the bilayer with bound vims was different from that without vims, resulting in a change of the optical properties of the polymer backbone. 

---