Oligomeric state

The infrared spectra of A-4-thiazoline-2-ones are characterized by a strong absorption around 1650 cm (55, 86, 103, 107. 870). For the N-H derivatives, the whole range 2700 to 3200 cm is covered by a strong absorption related to the dimeric and oligomeric states of the hydrogen-bonded structures (85, 86). 

Fairman, R., et al. Multiple oligomeric states regulate the DNA binding of helix-loop-helix peptides. Proc. Natl. Acad. Sci. USA 90 10429-10433, 1993. 

FIGURE 6.46 The oligomeric states of hemerythrin in various marine worms, (a) The hemerythrin in Thermkte zostericola crystallized as a monomer (b) the octameric hemerythrin crystallized from Phascolopsis gouldii (c) the trimeric hemerythrin crystallized from Sipiwnosoma collected in mangrove swamps in Fiji. 

Yeow, E. K. L. and Clayton, A. H. A. (2007). Enumeration of oligomerization states of membrane proteins in living cells by homo-FRET spectroscopy and microscopy Theory and application. Biophys. J. 92, 3098-104. 

These three examples emphasize the idea that a complete description of amyloid fibril polymorphisms will only be achieved when 3D structures at atomic detail become available (Luhrs et al., 2005). Last but not least, since the various morphologies observed for amyloid fibrils are defining the end point of the assembly process, an essential need is to properly define the early stages of fibril formation, namely the oligomeric states of the peptide or protein in solution prior to assembly into fibrils. 

The subsequent import mechanism has not been clarified, but it has a remarkable character it can pass proteins in a folded or even oligomerized state. 

Selected entries from Methods in Enzymology [vol, page(s)] Association constant determination, 259, 444-445 buoyant mass determination, 259, 432-433, 438, 441, 443, 444 cell handling, 259, 436-437 centerpiece selection, 259, 433-434, 436 centrifuge operation, 259, 437-438 concentration distribution, 259, 431 equilibration time, estimation, 259, 438-439 molecular weight calculation, 259, 431-432, 444 nonlinear least-squares analysis of primary data, 259, 449-451 oligomerization state of proteins [determination, 259, 439-441, 443 heterogeneous association, 259, 447-448 reversibility of association, 259, 445-447] optical systems, 259, 434-435 protein denaturants, 259, 439-440 retroviral protease, analysis, 241, 123-124 sample preparation, 259, 435-436 second virial coefficient [determination, 259, 443, 448-449 nonideality contribution, 259, 448-449] sensitivity, 259, 427 stoichiometry of reaction, determination, 259, 444-445 terms and symbols, 259, 429-431 thermodynamic parameter determination, 259, 427, 443-444, 449-451. 

The only measured enthalpy of vaporization is for n-butyl lithium, 107.1 2.9 kJmoU, from which the constant b(Li) in equation 4 is derived as 85 kJmoU. This is a hefty value compared to fc(OH) = 29, b(—S(0)2—) = 53 and b(CY) = 11 kJmoU. However, the oligomeric states of the organolithium liquid and gas phases are not known with certainty. This is reminiscent of problems for hydrogen-bonded species such as carboxylic acids and hydrogen fluoride. 

Size exclusion chromatography Purity and oligomeric state... 

Role of a and d Positions in Controlling Oligomerization State and Stability... 

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