Mass spectrometry to characterize

K.L.Johnson, T.D. Veenstra,J.M. Londowski, A.J.Tomlinson, R. Kumar, S. Naylor, On-line sample clean-up and chromatography coupled with electrospray ionization mass spectrometry to characterize the primary sequence and disulfide bond content of recombinant calcium binding proteins, Biomedical Chromatography 13 (1999)37-45. 

Global proteomic techniques are used for biological problems for which no molecular entry point is known. The idea is that changes in a cellular system can be understood better by analyzing its entire protein content quantitatively. The main analytical tools are a global and quantitative protein display and mass spectrometry to characterize the proteins that were identified for showing relevant quantitative changes. 

Su, J., Mrksich, M. Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates. Angew. Chem. Int. Ed. 2002, 41, 4715-4718. 

Monegier, B., Clerc, F.F., Van Dorsselaer, A., Vuihorgne, M., Green, B., Cartwright, T. (1991). Using mass spectrometry to characterize recombinant proteins. Part II. Pharm. Tech-nol., 15(4), 28 10. 

The use of mass spectrometry to characterize oligosaccharides is not new. Indeed, GC/MS has been used for several decades to identify monosaccharides or very small oligosaccharides. The use of GC/MS needs the prior derivatization of these molecules by methylation, acetylation or trimethylsilylation. 

Carre V, Aubriet F, Scheepers PT, Krier G, MuUer JF. Potential of laser ablation and laser desorption mass spectrometry to characterize organic and inorganic environmental pollutants on dust particles. Rapid Com mu n Mass Spectrom 2005 19 871-80 [Epub ahead of print]. 

Goodley, P. Moore, S. Sadilek, M. The Use of API-Ion Trap Mass Spectrometry to Characterize Biosynthesis Products, in Proceedings ofthe 47th ASMS Conference on Mass Spectrometry and Allied Topics. Dallas, Texas, June 13-17, 1999. 

Gavin et al. [13] made use of a combination of Tandem-Affinity purification (TAP) and mass spectrometry to characterize multiprotein complexes in S. cerevisiae. Ho et al. [14] identified protein complexes covering about 25% of the yeast proteome using a similar procedure, with a different purification step. 

Examples of polysiloxane degradation studies include the role of surfactants in suppressing aging of silica-PDMS gels, " the effects of pigments on the stability of montmorillonite-PDMS composites, and the use of NMR and mass spectrometry to characterize degradation processes. " " ... 

Karl, T., Jobson, T., Kuster, W.C., Williams, E., Stutz, I, Shelter, R., Hall, S.R., Goldan, R, Fehsenfeld, E, Lindinger, W. (2003) Use of proton-transfer-reaction mass spectrometry to characterize volatile organic compound sources at the La Porte super site during the Texas Air Quality Study 2000. Journal of Geophysical Research-Atmospheres, 108,4508. 

Regert, M. (2009) Direct mass spectrometry to characterize wax and lipid. In Organic Mass Spectrometry in Art and Archaeology, edited by Colombini, M.P., Modugno, F. Chichester, UK John Wiley Sons, pp. 97-129. 

Chia, V.K.F., Mount, G.R., Edgell, M.I, Magee, C.W. (1999) Recent advances in secondary ion mass spectrometry to characterize ultralow energy ion implants./cuirnaZ of Vacuum Science < Technology B Microelectronics and Nanometer Structures, 17, 2345-2351. 

Frisbie, C.D., Martin, J.R., Duff, R.R. Jr., Wrighton, M.S. (1992) Use of high lateral resolution secondary-ion mass spectrometry to characterize self-assembled monolayers on microfabricated structures. J. Am. Chem. Soc., 114, 7142-7145. 

Lin, S., Huang, M., Chang, H., Shiea, J. (2007) Using electrospray-assisted laser desorption/ionization mass spectrometry to characterize organic compounds separated on thin-layer chromatography plates. Analytical Chemistry, 79, 8789-8795. 

Bonchio, M., Licini, G Modena, G., Moro, S, Bortolini, O., Traldi, P and Nugent W,A. (1997) Use of electrospray ionization mass spectrometry to characterize chiral reactive intermediates in a titanium alkoxide mediated sulfoxidation reaction. Chem. Commun., 869-870. 

A study comparing plasma desorption (PD) and fast atom bombardment (FAB) mass spectrometry to characterize clusters of this type found that the isocyanide-capped clusters 30 showed strong molecular ion peaks by both methods. PD and FAB were also used to identify the molecular ions of dimers of trimers, ... 

Lin, S.Y. Huang, M.Z. Chang, H.C. Shiea, J. Using Electrospray-Assisted Laser Desorption/Ionization Mass Spectrometry to Characterize Organic Compounds Separated on Thin-Layer Chromatography Plates. Anal. Chem. 2007, 79, 8789-8795. 

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