Oligosaccharide characterization

Combining gel filtration, different glycosidases and different lectins for an analysis, the overall structures of oligosaccharides could be deduced. 

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V. Kovacik, J. Hirsch, P. Kovac, W. Heerma, J. Thomas-Oates, and J. Haverkamp, Oligosaccharide characterization using collision-induced dissociation fast atom bombardment mass spectrometry Evidence for internal monosaccharide residue loss, J. Mass Spectrom., 30 (1995) 949-958. 

Most results with ion-trap instrument were obtained nsing three-dimensional ion traps. A Q-LIT was recently introduced as well. Such a system shows great promise in oligosaccharide characterization. It provides collision-cell CID with ion-trap sensitivity but without low m/z cut-off, CID in the LIT giving MS possibilities, and... 

Oligosaccharides characterized by paper chromatography only, or presence of linkage inferred from other data (for example, cleavage products from methylated gum or derivative), or both. 

This year has seen a number of mass spectrometric applications to oligosaccharide characterizations. Uqmd secondary ion mass spectrometry (LSIMS) has been used in characterization of tetra-, hexa- and octasaccharides derived from porcine intestinal heparin. Laser desorption time-of-flight MS of heparin fragments complexed with (Arg-Gly)io and (Arg-Gly)is overcomes the problem... 

Gillece-Castro, B. L. Burlingame, A. L. Oligosaccharide Characterization with High-Eneigy Collision-Induced Dissociation Mass Spectrometry. Met. Enzy. 1990, 193, 689-712. 

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