Proteomics global

Lu B, Soreghan BA, Thomas SN, Chen T, Yang AJ. Towards global proteomic analysis in a PSl/APP mouse of Alzheimer s disease. ACS. San Diego, 2005. 

Cheung W, Darfler M, Alvarez H, et al. Apphcation of a global proteomic approach to archival precursor lesions deleted in malignant brain tumors 1 and tissue transglutaminase 2 are upregulated in pancreatic cancers. Pancreatology 2008 8 608-616. 

Global proteomic profiling by MS is gaining significant attention as a tool for discovering disease biomarkers. Two basic approaches have been explored. With the first, MS analysis is performed with a material from a specific disease condition and the mass spectra are compared to those of normal individuals or related disease conditions. SELDI-TOF MS gained popularity in this area because of its simplicity and the requirement for only small amounts of samples.38"13 In MS-pattem based disease categorization, the mass spectral patterns are considered reflective of the proteins present in samples from distinct clinical conditions. 

Global proteomic techniques are used for biological problems for which no molecular entry point is known. The idea is that changes in a cellular system can be understood better by analyzing its entire protein content quantitatively. The main analytical tools are a global and quantitative protein display and mass spectrometry to characterize the proteins that were identified for showing relevant quantitative changes. 

Proteomics can be divided into two major areas of research—global and targeted proteomics (Pandey and Mann 2000). Global proteomics compares protein expression in a cell, tissue, or body fluid in a comprehensive and global fashion. In this approach, proteins derived from a total cellular or tissue extract or from a body fluid are typically displayed on two-dimensional gels and... 

Fig. 2A, B Global (A) vs targeted (B) proteomics. In a global proteomics approach, all the protein constituents of a cell are isolated and subjected to 2D PAGE. In a targeted proteomics experiment, only a subset of proteins is analyzed. An example is the complex that is formed between a transmembrane receptor and cytoplasmic signaling proteins upon ligand activation of the receptor...

Radulovic D, Jelveh S, Ryu S, et al. Informatics platform for global proteomic profiling and biomarker discovery using liquid-chromatography-tandem mass spectrometry. Mol Cell Proteomics 2004 21 21. 

Combining affinity chromatography, two-dimensional electrophoresis, and mass spectroscopy, Becamel and colleagues pioneered a global proteomic approach to 5-HT receptosomes (Chapter 7). These groundbreaking studies employed either the entire or partial carboxyl terminus of the 5-HT2 receptors as bait and heralded the discovery of multiple FRAPs (14,15). Since then, the proteomic strategy has been successfully applied to other 5-HT receptor subtypes (16,17). 

Rosty C, Goggins M. Identification of differentially expressed proteins in pancreatic cancer using a global proteomic approach. Methods Mol Med 2005 103 189-197. 

One-step characterization of a eukaryotic cell pro-teome is difficult if not impossible to achieve. There is a growing trend in eukaryotes proteomics toward characterization of subcellular structures. The reason for this shift from global proteomics to subcellular proteomics is the complexity of eukaryotic cells and subcellular organelles. Therefore the proteomic analysis of subcellular organelles will be an important aspect of toxicoproteomics. 

Molina H, Horn DM, Tang N, Mathivanan S, Pandey A. Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectromefry. Proc Natl Acad Sci U. S. A. 2007 104 2199-2204. 

Wang, H. et al. (Goh et al. 2007) 2007. Characterization of mouse proteome using global proteomic analysis complemented with cysteinyl peptide enrichment. J. Proteome Res. 10, 1-13. 

Global proteomics describes the analysis of the combined set of all protein products encoded by a genome. It can determine not only expression but also sub-cellular localization and post-translational modifications of... 

Microfluidic devices that will enable global proteomic profiling will be particularly useful for biomarker discovery applications where the researcher is interested in comprehensively mapping all peptide/protein components in given biological fluids or tissues. Differential protein expression analysis will enable the discovery of specific protein markers or protein co-expression patterns that... 

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