Proteome, yeast

Karlberg O, Canback B, Kurland CG, Andersson SG (2000) The dual origin of the yeast mitochondrial proteome. Yeast 17 170-187... 

Gavin AC et al (2002) Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415 141-147... 

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

Hu, J. C. (2000). A guided tour in protein interaction space coiled coils from the yeast proteome. Proc. Natl. Acad. Sci. USA 97, 12935-12936. 

Breci, L., Hattrup, E., Keeler, M., Letarte, J., Johnson, R., Haynes, PA. (2005). Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing. Proteomics 5, 2018-2028. 

Graumann, J., Dunipace, L.A., Seol, J.H., McDonald, W.H., Yates, J.R., 3rd Wold, B.J., Deshaies, RJ. (2004). Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast. Mol. Cell. Proteomics 3, 226-237. 

Nagele, E., Vollmer, M., Horth, P. (2004). Improved 2D nano-LC/MS for proteomics applications a comparative analysis using yeast proteome. J. Biomol. Tech. 15, 134—143. 

Peng, J., Elias, J.E., Thoreen, C.C., Licklider, L.J., Gygi, S.P. (2003). Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC—MS/MS) for large-scale protein analysis the yeast proteome. J. Proteome Res. 2, 43-50. 

Washburn, M.P., Wolters, D., Yates, J.R., 3rd (2001). Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19, 242-247. 

Joubert R et al. Two-dimensional gel analysis of the proteome of lager brewing yeasts. Yeast 2000 16 511-522. 

MacKay, V. L., Li, X., Flory, M. R., Turcott, E., and Law, G. L. (2004). Gene expression analyzed by high-resolution state array analysis and quantitative proteomics Response of yeast to mating pheromone. Mol. Cell Proteomics 3, 478—489. 

Li, J., Steen, H., and Gygi, S.P. (2003) Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents. The yeast salinity stress response. Mol. Cell. Proteomics 2, 1198-1204. 

This multidimensional protein identification technology (MudPIT) specifically incorporates a strong cationic exchange (SCX) column in tandem with an RP column to achieve maximal resolution and exquisite sensitivity. MudPIT is effective for studying complex proteomes such as mammalian cellular samples. It has been applied to large-scale protein characterization with identification of up to 1484 proteins from yeast in a single experiment.12... 

L. Breci, E. Hattrup, M. Keeler, J. Letarte, R. Johnson, and P. A. Haynes. Comprehensive Proteomics in Yeast Using Chromatographic Fractionation, Gas Phase Fractionation, Protein Gel Electrophoresis, and Isoelectric Focusing. Proteomics, 5(2005) 2018-2028. 

J. Peng, et al., Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC-MS/MS) for Large-Scale Protein Analysis The Yeast Proteome. J. Proteome Res., 2, no. 1 (2003) 43-50. 

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