On-line Mass Spectrometry

The analyses of illicit drugs and metabolites in the sampled waters were performed following a previously described and validated fully automated method based on on-line solid phase extraction-liquid chromatography-tandem mass spectrometry (on-line SPE-LC-MS/MS) [19]. 

Mallet, C. R., Mazzeo, J. R., and Neue, U. (2001). Evaluation of several solid phase extraction liquid chromatography/tandem mass spectrometry on-line configurations for high-throughput analysis of acidic and basic drugs in rat plasma. Rapid Commun. Mass Spectrom. 15 1075-1083. 

The detection modes for a peptide map are the same as those described earlier for HPLC. Absorbance at low UV wavelengths is most often used. Absorbance at other wavelengths, tryptophan fluorescence, or fluorescence after derivatization might provide additional information on the location of a modification (see Section IV). The use of electrospray mass spectrometry on-line with HPLC is a powerful tool for peptide mapping that can identify the modification without further analysis.63... 

Mallet, C.R. Mazzeo, J.R. Neue, U. Evaluation of Several Solid Phase Extraction Liquid Chromatography/Tandem Mass Spectrometry On-line Configurations for High-throughput Analysis of Acidic and Basic Drugs in Rat Plasma," Rapid Commun. Mass Spectrom. 16,421 26 (2002). 

Hansel, A., Jordan, A., Holzinger, R. Proton transfer reaction mass spectrometry on-line trace gas analysis at the ppb level. Int. J. Mass Spectrom. Ion Proc. 149, 609-619, 1995. 

Edwards, E. and Thomas-Oates, J., Hyphenating liqnid phase separation techniques with mass spectrometry on-line or off-line. AnaZy f, 130, 13, 2005. 

The major question concerning methods for interfacing mass spectrometry on-line with chromatography is how to selectively discard the mobile phase while preserving as much as possible of the analyte, which is much more difficult for HPLC than for GC. 

Alberici, R.M., Mendes, M.A., Jardim, W.F., Eberlin, M.N. (1998) Mass Spectrometry On-Line Monitoring and MS Product Characterization of T1O2/UV Photocat-alytic Degradation of Chlorinated Volatile Organic Compounds. J. Am. Soc. Mass Spectrom. 9 1321-1327. 

Ebdon, L., Fisher, A., Handley, H., and Jones, P. (1993). Determination of trace metals in concentrated brines using inductively coupled plasma mass spectrometry on-line preconcentration and matrix elimination with flow injection./. Anal. Atomic Spectrom. 8(7), 979-982. 

Warneke, C., de Gouw, J. A., Lovejoy, E. R. et al. (2005) Development of proton-transfer ion trap-mass spectrometry on-line detection and identification of volatile organic compounds in air. / Am. Soc. Mass Spectrom. 16, 1316. 

Tiller, P. R., Yu, S., Castro-Perez, J., Fillgrove, K. L., and Baillie, T. A. (2008). High-throughput, accurate mass liquid chromatography/tandem mass spectrometry on a quadrupole time-of-flight system as a first-line approach for metabolite identification studies. Rapid Commun. Mass Spectrom. 22 1053-1061. 

Hansel, A., Jordan, A., Warneke, C. et al. (1998) Improved detection limit of the proton transfer reaction mass spectrometer on-line monitoring of volatile organic compounds at mixing ratios of a few PPTV. Rapid Communications in Mass Spectrometry, 12 (13), 871-5. 

Peptides were sequenced using either an Applied Biosystems Model 470 or 477 or a Hewlett Packard GIOOOA protein sequencer, each equipped with narrow bore RP-HPLC for on-line analysis of the PTH-amino acids. Mass analysis of peptides was performed using matrix-assisted laser desorption/ionization mass spectrometry on a KRATOS MALDl 111 with a-cyano-4 hydroxy-cinnamic acid as the matrix. 

Tegtmeyer D, Heindriehs A, Heitbaum J (1989) Electrochemical on line mass spectrometry on a rotating electrode inlet systean. Ber Bunsenges Phys Chem 93 201-206... 

Samples to be examined by inductively coupled plasma and mass spectrometry (ICP/MS) are commonly in the form of a solution that is transported into the plasma flame. The thermal mass of the flame is small, and ingress of excessive quantities of extraneous matter, such as solvent, would cool the flame and might even extinguish it. Even cooling the flame reduces its ionization efficiency, with concomitant effects on the accuracy and detection limits of the ICP/MS method. Consequently, it is necessary to remove as much solvent as possible which can be done by evaporation off-line or done on-line by spraying the solution as an aerosol into the plasma flame. 

The mass spectrometer (ms) is a common adjunct to a chromatographic system (see Mass spectrometry). The combination of a gas chromatograph for component separation and a mass spectrometer (gc/ms) for detection and identification of the separated components is a powerful tool, particularly when the data are collected usiag an on-line data-handling system. QuaUtative information inherent ia the separation can be coupled with the identification of stmcture and relatively straightforward quantification of a mixture s components. 

An on-line chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI/MS/MS) methods was developed for rapid screen of pharmacokinetics of different drugs, including 5 (98RCM1216). The electron impact mass spectrum of 5 and ethyl 9,10-difluoro-3-methyl-7-oxo-2,3-dihydro-7Ff-pyrido[l,2,3- fe]-l,4-benzoxazine-6-carboxylate was reported (97MI28). Electron impact/Fourier transform... 

A. J. Tomlinson and S. Naylor, Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry foi analysis of peptide mixtures, J. Capillary Electrophoresis 225-233 (1995). 

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