Mannan binding protein

Kozutsumi, Y, Kawasaki, T, and Yamashima, I (1980) Isolation and characterization of a mannan-binding protein from rabbit serum Biochem Biophys Res Commun 95, 658—664. 

Opanasopit, P., Shiraishi, K., Nishikawa, M., Yamashita, F., Takakura, Y. and Hashida, M. (2001) In vivo recognition of mannosylated proteins by hepatic mannose receptors and mannan-binding protein. Am. J. Physiol., in press. 

Nevens, J. R., Mallia, A. K., Wendt, M. W., and Smith, P. K. (1992). Affinity chromatographic purification of immunoglobulin M antibodies utilizing immobilized mannan binding protein. ]. Chromatogr. 597, 247-256. 

M. Terada, K.-H. Khoo, R. Inoue, C.-I. Chen, K. Yamada, H. Sakaguchi, N. Kadowaki, B. Y. Ma, S. Oka, T. Kawasaki, and N. Kawasaki, Characterization of oligosaccharide ligands expressed on SW1116 cells recognized by mannan-binding protein, J. Biol. Chem., 280 (2005) 10897-10913. 

MBP (mannan-binding protein) MASP-1 Protein that recognizes mannose residues on oligosaccharides on cell surfaces Homologous to C1 r C1 s, cleaves C1, C2 and C4 directly... 

IgM Purification Kit Pierce Mannan Binding Protein Affinity... 

Key words Affinity chromatography, Affinity matrices, Antigen-specific antibodies, Hapten-specific antibodies, Specie-specific antibodies, Cross-reacting immunoglobulin, Sepharose, Affigel, IgM, IgA, Mannan binding protein, Jacalin, Protein A, Protein G, Protein L... 

Protein L is another protein that can be used for antibody purification as it binds kappa light chains (see Note 4). Mannan Binding Protein (MBP) and jacalin (extracted from Jackfruit) bind fairly specifically to mouse or human IgM and human IgA... 

Bedair, M., and El Rassl, Z. (2004). Affinity chromatography with monolithic capillary columns I. Polymethacrylate monoliths with Immobilized mannan for the separation of mannose-binding proteins by capillary electrochromatography and nano-scale liquid chromatography. /. Chromatogr. A 1044, 177-186. 

First binding-protein biosensor immobilized concanavalin A in a polyvinyl chloride membrane on a platinum wire electrode to measure yeast mannan [11]... 

Biosorption is a rather complex process affected by several factors that include different binding mechanisms (Figure 10.4). Most of the functional groups responsible for metal binding are found in cell walls and include carboxyl, hydroxyl, sulfate, sulfhydryl, phosphate, amino, amide, imine, and imidazol moieties.4 90 The cell wall of plant biomass has proteins, lipids, carbohydrate polymers (cellulose, xylane, mannan, etc.), and inorganic ions of Ca(II), Mg(II), and so on. The carboxylic and phosphate groups in the cell wall are the main acidic functional groups that affect directly the adsorption capacity of the biomass.101... 

Ellipsometry can follow the interactions between two types of biological macromolecules, the first of those two bound physically to the surface, the other acting from the solution. The binding of conconavalin A to adsorbed mannan 180) and of cholera toxin to adsorbed ganglioside t83) are examples. The adsorption of complement factors to an antibody-coated surface was monitored by ellipsometry and a modification of the same method was used for quantification of migration inhibition of human polymorphonuclear leucocytes 182). Interaction of proteins and cells with affinity ligands covalently coupled to silicon surfaces has been also studied 183). 

The a-(1 3)-hnked D-mannans of Nothogenia fastigiata sulfated on C-2 and C-6 also mimic the minimal binding domain of HS. However, the lower antiviral activity of the fractions with D-xylose side chains suggests that branching precludes binding to the gC viral protein [21],... 

Metal analysis revealed one atom of Mn2+ and six atoms of Ca2+ associated with each 66.5 kg of protein.142 Addition of 5 mM Ca2+ and mM Mn2+ to the native lectins enhanced both hemagglutination and mannan precipitation. This indicates that either some metal loss occurred during purification, or that the metal binding-sites are never completely occupied442 (compare Refs. 300 and 301). Mn2+ stimulated the hemagglutinating activity of all lentil-lectin preparations more than did Ca2+, whereas Ca2+ promoted mannan precipitation more effectively.442 Demetallization, by dialysis against 0.1 M EDTA followed by M acetic... 

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