Macrophage stimulating protein characterization

Several other cell types have also been shown to secrete histamine-releasing activity, some of which may be peptide in nature (although more work is necessary for a definitive characterization). For example, human lung macrophages cultured for 24 h have been shown to release a soluble factor (12 and 30 kDa) that stimulates isolated human lung mast cells and human basophils to release histamine [ 145]. The generation and release of this factor developed over time (> 1 h) and was blocked by cycloheximide, indicating that protein... 

In the first differential proteomics approach for the identification and characterization of proteins involved in both the inflammatory cascade and Leflunomide mode of action (Dax et al, 1998), the murine monocyte/macrophage cell line RAW 264.7 was employed and the RAW 264.7 proteomes of normal, unstimulated RAW 264.7 macrophages with the respective LPS-stimulated and/or drug-treated were compared. This cell line was chosen since RAW 264.7 cells have been used as a model system to study immune responses (Bahl et al, 1994) successfully before. 

A. Tsarbopoulos, B. N. Pramanik, J. Labdon, P. Reichert, G. Gitlin, S. Patel, V. Sardana, T. L. Nagabhushan, and P. P. Trotta, Isolation and characterization of a resistant core peptide of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) confirmation of the GM-CSF amino acid sequence by mass spectrometry. Protein Sci. 2 (1993), 1948-1958. 

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