Lymphocyte membrane

Lymphocyte Membrane Protection by Antioxidants against N02 Cell Membrane Destruction Is Shown by Cell Staining with Eosin... 

Maczek C, Bock G, Jurgens G, Schonitzer D, Dietrich H, Wick G (1998) Environmental influence on age-related changes of human lymphocyte membrane viscosity using severe combined immunodeficiency mice as an in vivo model. Exp Gerontol 33(5) 485—498... 

So far three subtypes of ft receptors have been identified and cloned. They differ in their distribution, the Pi type being found in the heart, the P2 in lung, smooth muscle, skeletal muscle and liver, while the P type occurs in adipose tissue. There is evidence that P2 adrenoceptors occur on the lymphocyte membrane also but the precise function there is unknown. 

Increased density of 5-HT2a receptors on the platelet membrane Decreased uptake of 3H-5-HT into platelet Increased beta adrenoceptor density on lymphocyte membrane Increased density of alpha-2 adrenoceptors on platelet membranes ) Blrmted growth hormone response to a clonidine challenge Blrmted prolactin response to a fenfluramine challenge... 

Mild, selective oxidation of the sialic acid side-chain, followed by the addition of bisulfite and Toluidine Blue has been used for topo-optical staining of sialoglycoconjugates in erythrocyte and lymphocyte membranes. This method, which is based on a polarization-microscope technique, pennits not only specific, and very sensitive, light-microscope staining of sialic acid residues on cell surfaces but also the study of the spatial arrangement of sialoglycoconjugate molecules on cell surfaces.192,197-198... 

Loor, F., Fomi, L., Pemis, B. (1972). The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur. J. Immunol. 2,203-212. 

Salsano, F., Froland, S.S., Natvig, J.B., Michaelsen, T.E. (1974). Same idiotype of B-lymphocyte membrane IgD and IgM. Formal evidence for monoclonality of chronic lymphocytic leukemia cells. Scand. J. Immunol. 3,841-846. 

Vassalli, P., Tedghi, R., Lisowska-Bemstein, B., Tartakoff, A., Jaton, J.-C. (1979). Evidence for hydrophobic region within heavy chains of mouse B lymphocyte membrane-bound IgM. Proc. Natl. Acad. Sci. USA 76,5515-5519. 

Increased beta adrenoceptor density on lymphocyte membrane... 

These same surface glycoproteins have been modeled as receptors for antibody molecules and in the binding of lectin molecules, such as concanavalin A. On the lymphocyte membrane,... 

Miguet, L., Pacaud, K., Felden, C., Hugel, B., Martinez, M.C., Freyssinet, J.M., Herbrecht, R., Potier, N., van Dorsselaer, A. and Mauvieux, L. (2005) Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization. Proteomics 6, 153-171. 

Allan, Auger and Crumpton used Con A-Sepharose to isolate glycoprotein cell surface receptors for concanavalin A from pig lymphocyte membranes solubilized with sodium deoxycholate [134]. 

Cyclosporine provides maintenance immunosuppression by inhibition of the activation of T lymphocytes via a multifaceted mechanism. The drug, a fat soluble 11 amino acid cyclic polypeptide, crosses the lymphocyte membrane freely where it forms a pharmacologically active complex with the intracellular immunophilin receptor cyclophilin. This complex, but not cyclosporine by itself, inhibits the Ca /calmodulin-activated form of serine/threonine phosphatase calcineurin, thereby inhibiting the activation of NFAT cells. The latter action is considered to be the key step... 

Frazier DE, Tarr MJ, Olsen RG. 1992. Evaluation of murine lymphocyte membrane potential, intracellular free calcium, and interleukin-2 receptor expression upon exposure to 1,1-dimethylhydrazine. Toxicol Lett 61 27-37. 

Pernis, B. (1985) Internalisation of lymphocyte membrane components. Immunol. 

Cholesterol depletion of T lymphocyte membranes also inhibits the immune response, i.e. cell-mediated cytotoxicity [102]. Cholesterol enrichment or depletion inhibits Con A-stimulated transformation of lymphocytes [87,103]. However, functions dependent on protein synthesis may be inhibited in cholesterol-depleted cells, not primarily because of the loss of cholesterol, but because of the secondary loss of K+. 

Corticosteroids stabilize lymphocytic membranes, reducing the production of immunoglobulins and their compliment. Lymphocyte production is also inhibited, producing lymphocytopenia. 

Solubilized surface proteins from normal human lymphocytes have been obtained by mild digestion with trypsin, and the binding of these components to labelled Ricinus sanguineus has been studied.A method for the direct visualization of lymphocyte membrane receptors has made use of fluoresceinyl derivatives of glycosylated cytochemical markers such as bovine serum albumin covalently attached to either D-mannose, 2-acetamido-2-deoxy-D-galactose, L-fucose, or lactose. 

In contrast to human membrane-derived immunoglobulins M and D, those from murine lymphocyte membrane display different affinities for lentil lectin. 

---