Lupin ELISA

The present volume reflects these developments, and there is a growing emphasis on bioactive natural products. Articles in this volume include those on structure-activity relationships of highly sweet natural products, chemical constituents of cchinodenns, diterpenoids from Rabdosia and Eremophila sp., structural studies on saponins, marine sesquiterpene quinoncs and antimicrobial activity of amphibian venoms. The reviews on bioactive metabolites of Phomopsis, cardenolide detection by ELISA, xenocoumacins and bioactive dihydroisocoumarins, CD studies of carbohydrate-molybdate complexes, oncogene function inhibitors from microbial secondary metabolites and Gelsemium and Lupin alkaloids present frontier developments in several areas of natural product chemistry. It is hoped that the present volume, which contains articles by eminent authorities in each field, will be received with the same enthusiasm as the previous volumes of this series. 

FIGURE 22.10 (a) Immunoblot using polyclonal anti-lupin antibody (1, molecular-weight marker 2, L. aftMj-derived extract 3, L. angustifolius flour extract) (b) Representative linear six-point calibration curve based on the standard curve obtained using the lupin protein standard in the sandwich ELISA. (From Holden etal., 2005.) (Reprinted with permission from Reference Citation. Copyright 2005 American Chemical Society.)... 

Furthermore, a novel polyclonal/monoclonal-based sandwich ELISA for the detection of lupin proteins in foods was described (Holden et al., 2007). The monoclonal antibody reacts specifically with a-conglutin (Dooper et al., 2007). The assay has a detection limit of Img protein/kg food and is sensitive to both native and processed proteins from L. angustifolius and L. albus. A selection of 112 food samples, both with and without lupin declaration, was analyzed showing that the majority of products were labeled correctly. 

Holden L, Ereste CK, Egaas E (2005). Quantitative sandwich ELISA for the determination of lupine (Lupinus spp.) in foods. J. Agric. Food Chem., 53 5866-5871. 

Holden L, Moen LH, Sletten GB, Dooper MM (2007). Novel polyclonal-monoclonal-based ELISA utilized to examine lupine (Lupinus species) content in food products. J. Agric. Food Chem., 55 2536-2542. 

Allen, Greirson, and Harris, from the Agricultural Chemistry Laboratory of the Chemistry Centre in Western Australia, use the following steps for lupine alkaloid extraction in ELISA detection ... 

Allen DG, Greirson BN, Harris DJ. An enzyme immunosorbent assay (ELISA) for lupin alkaloids Comparison with gas chromatography. In von Baer D, editOT. International lupin Association sixth international lupin conference proceedings. November 25—30, 1990. Temuco-Pucon, Chile Asociacion Chilma del Lupino 1991. p. 406. 

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