Liver cell plates

Fig. 24.10 Lues connata Severe inflammatory infiltration (especially left of picture) sinusoidal fibrosis irregular liver cell plates (far right of picture). So-called interstitial syphilitic hepatitis (HE)...

Figure 1 Diagram illustrating the basic anatomical unit of the liver, the liver lobule, showing (1) the radial disposition of the liver cell plates and sinusoids around the central vein, (2) the centripetal flow of blood from branches of fhe hepafic artery and portal vein, and (3) the centrifugal flow of bile (small arrows) fo fhe small bile duct in the portal space. (Reproduced from Bloom W and Fawcett DW (eds.) (1968) A Textbook of Histology, 9th edn. Philadelphia Saunders redrawn and modified from Ham, Textbook of Histology. Philadelphia Lippincott, with permission from Lippincott.)...

The first batch of cells consisted of AC 133+ cells cultivated in the diffusion chambers submerged on top of the feeder (feeder -AC 133 cells /Fl-C). The second batch consisted of human embryonic liver cell suspension directly cocultured with AC133+ cells at equal initial quantities (5x10 ) in the diffusion chambers submerged in the 6-well plates without additional feeder layer (FC-C). Third batch of experiments represented AC133+ cells cultured in the DC surrounded by FL condition media (condition media-AC133+ cells/CM-C). In the control group cells were cultivated in the same condition without any additions and without feeder layers. 

In an application of the method to the detection of murine hepatocytes that secrete albumin, the authors employed SRBC coated with purified antibody against mouse serum albumin and plated such cells together with varying numbers of teased liver cells exactly as described for the hemolytic plaque assay. After the initial incubation, anti-mouse serum albumin... 

E. Hering proposed that the bile capillaries were bounded solely by liver cells and that the parenchyma had a structure of little plates rather than a trabecular form. 

Fig. 2.11 Diagram of the traditional ( classic ) hepatic lobule according to the lobular structure (F. Kiernan, 1833) (s. fig. 1.18) and as stereogram (H. Elias, 1949) the liver cell columns run radially from the limiting plate to the central vein (10) (s. fig. 2.16)...

Periportal inflammation Periportal hepatitis is characterized by penetration of the limiting plate. The border between the portal field and the lobule can appear irregular sometimes it assumes the shape of a maple leaf In this periportal zone (i.e. zone 1), piecemeal necroses may develop. They are, however, not true necroses , but apoptoses. Today, periportal inflammation with piecemeal necrosis is termed interface hepatitis. This condition is not always accompanied by piecemeal necrosis the inflammatory infiltrate can also enter the lobule without causing liver cell necroses. The composition of the inflammatory infiltrates is similar to that in the portal fields. Periportal infiammation contributes to the grading of chronic hepatitis, (l)... 

TIMP). Both factors reduce further degradation of connective tissue. The hepatocytes are now multilayered instead of normal single-layered cell plates and lose their microvilh. Fenestration of the sinusoids disappears, whereas the sinusoidal extracellular matrix increases, leading to capillarization of the sinusoids, (s. pp 406, 526) In this way, the distance between the hepatocytes and the blood becomes greater, and the clearance of macromolecular substances is reduced. Stronger flow resistance in the liver leads to portal hypertension. Portoportal and portocentral bands of connective tissue form, in which portosystemic intrahepatic shunts develop. 

Schematic representation of the radial architecture of the plates formed by the liver cells. Blood from the portal vein and the hepatic artery flows into sinusoids and eventually enters the central vein. The bile canaliculi are located between the liver cells. Bile flows in the opposite direction and empties into the bile duct in portal triads. [Reproduced with permission from A. W. Ham Histology, 8th ed., J. B. Lippincott, Philadelphia, 1979.]...

It also is possible to use intact ceils as a source of xenobiotic metabolizing enzymes. Activation for tissue culture systems can be provided by primary rat liver cells and irradiated hamster embryo cells which are plated as a feeder layer for the indicator cells. In the case of compounds which yield a spec-... 

Hepatocytes were prepared from livers of 14 day old chick-embryos [4]. The livers were cut into small pieces with scissors and incubated for 30 min at 37in Ca and Mg free Krebs-Hanselit buffer at pH 7.6, This was followed by a second 30 min. incubation in the presence of collagenase (0.5mg/ml) and hyaluronidase (Img/ml). At the end of the incubation period 3 volumes of M-199 medium were added and the free cells collected by centrifugation. The isolated cells were washed 3 times with M-199. They were finally suspended in M-199 in presence of 10% new-born calf serum and plated (Nunc 9 cm diameter plates 1.5 livers per plate). After 18 hr the medium and unattached cells were removed. The adherent cells were washed and incubated in fresh M-199 medium. or labelled precursors were... 

The Chang liver cells are cultured in parafBn-sealed 96-weU miCTolitre plates (24 h). Deficient outgrowth of fusiform or spindle-shaped cells is used as a criterion of cyto-inhibition (Ekwall and Sandstrom, 1978), after the cells have been in contact with the test substance. 

Newt, Triturus cristatus, adults, held in tank with a zinc-plated base South African clawed frog, Xenopus laevis 200 to 3000 overa 7-day period Zinc-poisoned newts were lethargic, ate poorly, and had skin darkening prior to death. Zinc residues were elevated in kidney, brain, liver, and intestine, when compared to controls. The hippocampus region of the brain of poisoned newts contained zinc-rich cells 82... 

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