Chang liver cells

C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells. Eur J Pharmacol 557 221-229. 

Kaviarasan, S., Ramamurty, N., Gunasekaran, P., Varalakshmi, E. and Anuradha, C.V. (2006) Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in Chang liver cells. Alcohol and Alcoholism 41 (3), 267-273. 

Bakardjieva, A., Galla, H. J., and Helmreich, E. J. M., Modulation of the /8-receptor adenylate cyclase interactions in cultured change liver cells by phospholipid enrichment. Biochem. 18, 3016 (1979). 

In addition, 10-membered lactone compounds, namely, decarestrictines, were identified by chemical screening for inhibition of cholesterol biosynthesis using in vitro and in vivo assay systems. 3(3-Hydroxy-l,ll-dioxo-ergosta-8,24(28)-diene-4a-carboxylic acid was isolated as an inhibitor of cholesterol biosynthesis using human Chang liver cell system. 

GSPE Increases bcl-2 expression and decreases p53 and c-myc expression normal human Chang liver cells Joshi, S.S. et al., Curr. Pharm. BiotechnoL, 2,187, 2001... 

Mitogen-activated protein kinases (MAPKs) mediate apoptosis and cell growth, and jim N-terminal kinase (JNK) in particular is activated by oxidative stress. Yang et al. (2008) suggested that JNKs/SAPKs and p38 MAPK are classic oxidative stress-activated protein kinases, and Kim et al. (2004) observed that treatment with selenite increased intracellular reactive oxygen species (ROS) levels and JNKl phosphorylation in Chang liver cells. 

A dichloromethane-methanol-water extract of Madagascar periwinkle demonstrated toxicity in Chang liver cells and adipose cells at concentrations of 12.5 fig/ml (van de Venter etal.2008). 

Eliasson, E. 1967. Repression of arginase synthesis in Chang liver cells. Exp. Cell Res., 48 1. 

Cytotoxicity tests, using a range of tissue cultures, would seem to provide a system more closely related to the whole animal. Baby hamster kidney cells, and human cells lines such as HEp-11, which are especially sensitive to trichothecenes, and Chang liver cells, have been successfully used for screening for the presence of toxic metabolites in food extracts. 

WEHT and to evaluate the protective effect of WEHT and its active phenolic compounds on oxidative damage in Chang liver cells. 

Sun-dried Hsian-tsao Mesona procumbens Hemsl.) (harvested in 1995) was obtained from a local market in Hua-lian county, Taiwan. The Chang liver cell line (CCRC 60024) was obtained from the Food Industry Research and Development Institute (Hsinchu, Taiwan). Protocatechuic acid, />-hydroxy-benzoic acid, caffeic acid, vanillic acid, syringic acid, kaempferol and apigenin were purchased from Sigma Chemical Co. (St. Louis, MO). All other chemicals and cultural medium were of reagent grade. 

Cytotoxicity of WEHT and Isolated Phenolic Compounds in Chang Liver Cells... 

The cytotoxicity of WEHT in Chang liver cells was evaluated based on its effect on cell growth (MTT test). Cell viability was greater than 95% when WEHT (0-1000 ig/mL) was incubated with cells at 37 C for 24 h. In addition, no cytotoxicity was found when Chang liver cells were treated with isolated phenolic compounds of Hsian-tsao under a concentration range of 0-100 pM (data not shown). 

Table I. Effect of Water Extract of Hsian-tsao on H202-induced Production of Lipid Oxidation Formation in Chang Liver Cells...

Figure 2. Inhibitory effect of phenolic compounds on H20rinduced lipid oxidation of Chang liver cells. A control containing no added sample represents 100 % lipid peroxidation.

Figure 3. Effect of water extract of Hsian-tsao (WENT) on H2O2 -induced DNA damage of Chang liver cells. p < 0.05 when compared with the control.

Figure 4. Genotoxicity of Chang liver cells treated with phenolic compounds. DNA strand breaks were detected by the comet assay. The concentration of sample was 10 pM. ° Values in each column with different letters are significantly different (p < 0.05).

Figure 6. Effect of water retract of Hsian-tsao (WEHT) pretreatment on hydrogen peroxide-induced intracellular ROS level in Chang liver cells. Chang liver cells was preincubated with sample for 20 h before analysis.

The Chang liver cells are cultured in parafBn-sealed 96-weU miCTolitre plates (24 h). Deficient outgrowth of fusiform or spindle-shaped cells is used as a criterion of cyto-inhibition (Ekwall and Sandstrom, 1978), after the cells have been in contact with the test substance. 

Giuliano, M. Bellavia, G. Lauricella, M. D Anneo, A. Vassallo, B. Vento, R. Tesoriere, G. Staurosporine-induced apoptosis in Chang liver cells is associated with down-regulation of Bcl-2 and Bcl-XL. Int. J. Mol. Med. 2004,13,565-571. 

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