Hemolytic plaque assay

The modified cells are washed twice more with the bicarbonate solution and are ready for use as target cells in the hemolytic plaque assay. 

An essential control is one for the specificity of plaque formation. In the example cited this consists of plates in which trinitrophenylated serum albumin (100 /u,g/ml) is incorporated into the top layer agarose as a specific inhibitor. Pl2iques forming in these plates are presumed to be anti-SRBC, and correction is made by subtracting their number. Another essential control omits complement. This description of the plaque assay is lacking in some details but it will suffice to introduce the reverse hemolytic plaque assay, which probably will be more relevant to the readers interests. 

In an application of the method to the detection of murine hepatocytes that secrete albumin, the authors employed SRBC coated with purified antibody against mouse serum albumin and plated such cells together with varying numbers of teased liver cells exactly as described for the hemolytic plaque assay. After the initial incubation, anti-mouse serum albumin... 

Antibody producing cells to sheep erythrocytes (SRBC) were measured by the hemolytic plaque assay as modified by Cunningham and Szenberg (1968). Mice were inoculated i.p. with 5 x 10 SRBC on day 0, and with 25 mg/kg pyran (NSC 46051) i.v. on days — 1, 0, 1 or 2 with respect to SRBC and the splenic plaque forming cells determined on day 3,4, 5 and 6 post SRBC inoculation. 

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